Phagocytosis of Leishmania Major Parasites by Murine Macrophages; what is happening under a Microscope?
Student Research in Translational Medicine,
Vol. 6 (2024),
24 Bahman 2024
Abstract
The promastigotes of Leishmania major (MRHO/IR/75/ER) were grown as previously described [1]. J774A.1 which is a murine macrophage Cell Line was purchased from Pasteur Institute of Iran and cultured using Roswell Park Memorial Institute medium (RPMI-1640) (Biowest, France) enriched with 10% heat-inactivated Fetal Bovine Serum (FBS) (Gibco, USA) with 2 mM L.glutamine (Sigma, Australia) and 1% penicillin/streptomycin (Gibco, USA). 7 × 106 macrophage cells were seeded in a T75 cell culture flask (SPL life sciences, South Korea). After 24 hours, 7 × 107 stationary phase promastigotes of L. major were added to the T75 flask containing J774A.1 macrophage cells. The video was recorded immediately and 3 hours after the infection of macrophages with L. major parasites under an inverted microscope (OPTIKA, Italy) with Samsung A13 mobile phone camera (Samsung, USA).
- Leishmania
- Leishmania major
- Macrophages

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References
Asadpour A, Riazi-Rad F, Khaze V, Ajdary S and Alimohammadian MH.Distinct strains of Leishmania major induce different cytokine mRNA expression in draining lymph node of BALB/c mice.Parasite Immunology.2013;35(1):42-50.
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