Cytotoxic Effects of Iranian Mistletoe Extract on a Panel of Cancer Cells Cytotoxicity of Mistletoe
Iranian Journal of Pharmaceutical Sciences,
دوره 2 شماره 3 (2006),
1 تموز 2006
,
صفحه 157-162
https://doi.org/10.22037/ijps.v2.39739
چکیده
Extracts derived from Viscum album have been shown to kill cancer cells in vitro. Some studies have noted that different species of this plant collected from around the world displayed cytotoxic effects in different extents. In the present study, we evaluated the effects of Iranian mistletoe extracts on five cancer cell lines. Plants growing on hornbeam tree (Carpinus betulus) were collected, air-dried and hydroalcoholic (MeOH-H2O with 2% acetic acid) and methanolic extracts were obtained using percolation. Also the plant juice was obtained by pressing. Cytotoxicity of the extracts on a panel of cancer cells (Hela, KB, MDA-MB-468, K562 and MCF- 7) were studied using colorimetric MTT assay. Results showed that plant juice was the most cytotoxic fraction on all cancer cells tested (IC50=0.0316 mg). The IC50 of hydroalcoholic and methanolic extracts were 0.1 and 0.316 mg, respectively. These results suggest that alkaloids and huge compounds like viscotoxin and lectins extracted by press or hydroalcoholic solvents were probably responsible for their cytotoxicity. Results also indicated that Hela cells were more resistant while KB cells were more sensitive to the cytotoxic effects of the extracts. It can be concluded that cytotoxicity of Iranian mistletoe extract on the cell lines tested closely depends on the host tree and extraction methods.
- Cancer cells
- Cytotoxicity
- Mistletoe
- MTT assay
- Viscum album
ارجاع به مقاله
مراجع
[2] Mengs U, Gothel D, Leng-Peshlow E. Mistletoe extracts standardized to mistletoe lectins in oncology: Review on current status of preclinical research. Anticancer Res 2002; 22: 1399-407.
[3] Samtleben R, Hajto T, Hostanska K. Mistletoe lectins as immunostimulants (chemistry, pharmacology and clinic). In: Wagner H, (editor). Immunomodulatory agents from plants. Basel, Switzerland: Birkhauser Verlag, 1999, pp. 223-41.
[4] Schrader G, Apel K. Isolation and characterization of cDNAs encoding viscotoxins of mistletoe (Viscum album). Eur J Biochem 1991; 198: 549- 53.
[5] Friess H, Beger HG, Kunz J, Funk N, Schilling M, Buchler MW. Treatment of advanced pancreatic cancer with mistletoe: Results of a pilot trial. Anticancer Res 1996; 16: 915-20.
[6] Grossarth-Maticek R, Kiene H, Baumgartner SM, Ziegler R. Use of Iscador, an extract of European mistletoe (Viscum album), in cancer treatment: prospective nonrandomized and randomized matched-pair studies nested within a cohort study. Altern Ther Health Med 2001; 7: 57-66, 68-72, 74-6.
[7] Sweeney EC, Palmer RA, Pfuller U. Crystallization of the ribosome inactivating protein ML1 from Viscum album (mistletoe) complexed with beta-D-galactose. J Mol Biol 1993; 234: 1279-81.
[8] Gabius HJ, Darro F, Remmelink M, Andre S, Kopitz J, Danguy A, Gabius S, Salmon I, Kiss R. Evidence for stimulation of tumor proliferation in cell lines and histotypic cultures by clinically relevant low doses of the galactoside-binding mistletoe lectin, a component of proprietary extracts. Cancer Invest 2001; 19: 114-26.
[9] Zee-Cheng RK. Anticancer research on Loranthaceae plants. Drug Future 1997; 22: 519-30.
[10] Kaegi E. Unconventional therapies for cancer: 3. Iscador. Task force on alternative therapies of the Canadian breast cancer research initiative. CMAJ 1998; 158: 1157-9.
[11] Gorter RW. Iscador. Congers, New York 10920, USA, 1998.
[12] Maier G, Fiebig HH. Absence of tumor growth stimulation in a panel of 16 human tumor cell lines by mistletoe extracts in vitro. Anticancer Drugs 2002; 13: 373-9.
[13] Burger AM, Mengs U, Schuler JB, Fiebig HH. Anticancer activity of an aqueous mistletoe extract in syngeneic murine tumor models. Anticancer Res 2001; 21: 1965-8.
[14] Gorter RW, Joller P, Stoss M. Cytokine release of a keratinocyte model after incubation with two different Viscum album L extracts. Am J Ther 2003; 10: 40-7.
[15] Ghassemi-Dehkordi N, Taleb AM. Isolation, identification and determination of medicinal plants constituents. Isfahan: Isfahan University of Medical Sciences Publication, 2001.
[16] Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation assays. J Immunol Methods 1983; 65: 55-63.
[17] Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB. Evaluation of a tetrazoliumbased semiautomated colorimetric Assay: assessment of
hemosensitivity testing. Cancer Res 1987; 47: 936-42.
[18] Bussing A, Schietzel M. Apoptosis-inducing properties of Viscum album L. extracts from different host trees, correlate with their content of toxic mistletoe lectins. Anticancer Res 1999; 19: 23-8.
[19] Yoon TJ, Yoo YC, Kang TB, Shimazaki K, Song SK, Lee KH, Kim SH, Park CH, Azuma I, Kim JB. Lectins isolated from Korean mistletoe (Viscum album var. coloratum) induce apoptosis in tumor cells. Cancer Lett 1999; 136: 33-40.
[20] Wagner H, Jordan FB. Studies on the standardization of mistletoe preparations. Oncology 1986; 43: 16-22.
[21] Stein GM, Berg PA Characterisation of immunological reactivity of patients with adverse effects during therapy with an aqueous mistletoe extract. Eur J Med Res 1999; 4: 169-77.
[22] Sweeney EC, Palmer RA, Pfuller U. Crystallization of the ribosome inactivating protein ML1 from Viscum album (mistletoe) complexed with beta-D-galactose. J Mol Biol 1993; 234: 1279-81.
[23] Stettin A, Schultze JL, Stechemesser E, Berg PA. Anti-mistletoe lectin antibodies are produced in patients during therapy with an aqueous mistletoe extract derived from Viscum album L. and neutralize lectin-induced cytotoxicity in vitro. Klin Wochenschr 1990; 68: 896-900.
[24] Lyu SY, Park SM, Choung BY, Park WB. Comparative study of Korean (Viscum album var. coloratum) and European mistletoes (Viscum album). Arch Pharm Res 2000; 23: 592-8.
[25] Kuttan G. Tumoricidal activity of mouse peritoneal macrophages treated with Viscum album extract. Immunol Invest 1993; 22: 431-40.
[26] Jurin M, Zarkovic N, Hrzenjak M, Ilic Z. Antitumorous and immunomodulatory effects of the Viscum album L. preparation Isorel. Oncology 1993; 50: 393-8.
[27] Timoshenko AV, Gabius HJ. Efficient induction of superoxide release from human neutrophils by the galactoside-specific lectin from Viscum album. Biol Chem Hoppe Seyler 1993; 374: 237- 43.
[28] Timoshenko AV, Dubovskaya LV, Karvatskaya OD, Zharkov VV, Gabius HJ. NO-dependent regulation of lectin- and menadione-induced H2O2 production by cells from pleural effusions of lung cancer patients and by immune cells. Int J Oncol 1999; 14: 793-8.
[29] Bussing A, Stein GM, Wagner M, Wagner B, Schaller G, Pfuller U, Schietzel M. Accidental cell death and generation of reactive oxygen intermediates in human lymphocytes induced by thionins from Viscum album L. Eur J Biochem 1999; 262: 79-87.
[30] Bocci V. Mistletoe (Viscum album) lectins as cytokine inducers and immunoadjuvant in tumor therapy: A review. J Biol Regul Homeost Agents 1993; 7: 1-6.
[31] Citores L, Ferreras JM, Iglesias R, Carbajales ML, Arias FJ, Jimenez P, Rojo MA, Girbes T. Molecular mechanism of inhibition of mammalian protein synthesis by some four-chain agglutinins. Proposal of an extended classification of plant ribosome-inactivating proteins (rRNA Nglycosidases). FEBS Lett 1993; 329: 59-62.
[32] Hajto T, Hostanska K, Weber K, Zinke H, Fischer J, Mengs U, Lentzen H, Saller R. Effect of a recombinant lectin, Viscum album agglutinin on the secretion of interleukin-12 in cultured human peripheral blood mononuclear cells and on NKcell-mediated cytotoxicity of rat splenocytes in vitro and in vivo. Nat Immun 1998; 16: 34-46.
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