Diversity of Phenolic Profiles in Peel of an Iranian Pomegranate Cultivar (Punica granatum L.) Phenolic Profiles in Peel of Iranian Pomegranate
Iranian Journal of Pharmaceutical Sciences,
Vol. 17 No. 1 (2021),
15 January 2021
,
Page 51-68
https://doi.org/10.22037/ijps.v17.40299
Abstract
Pomegranate (Punica granatum L.) is one of the oldest known fruits native to Iran. In addition to multiple studies carried out on different parts of pomegranate, the peels are noted due to various phytochemicals in different colors and regions. In the present study, major anthocyanins and non-anthocyanins of an Iranian black pomegranate cultivar (i.e., pomegranate samples of Ghaemshahr, Iran) were analyzed using high-performance liquid chromatography-mass spectrometry. In total, 30 compounds were obtained from pomegranate peels. Among these compounds, 5 anthocyanins, 1 gallotannin, 15 ellagitannins, 4 hydroxybenzoic acids, 2 gallagyl esters, 1 dihydroflavonol, and 7 hydroxycinnamic acids were identified based on their fragmentation patterns and ultraviolet spectra. Among the varieties of Iranian pomegranates, the anthocyanins of peonidin-hexoside and cyanidin-pentoside were identified in this species for the first time. Peonidin-hexoside, caffeic acid, coumaric acid-hexoside, and galloyl-hexoside were major phenolic compounds. In addition, the antioxidant activity of different fractions was tested using DPPH free radical scavenging in vitro assay (IC50: 133.13-557.23 μg/mL). The obtained results of this study highlighted that this cultivar can be an important candidate for future new drug discoveries.
- Punica granatum
- peel
- anthocyanin
- non-anthocyanin phenolic compounds
- HPLC-MS/MS
- antioxidant
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References
[2] Çam M, İçyer NC, Erdoğan F. Pomegranate Peel Phenolics: Microencapsulation, Storage Stability and Potential Ingredient for Functional Food Development. Food Sci. Technol. (2014) 55(1): 117-23.
[3] Singh B, Singh JP, Kaur A, Singh N. Phenolic Compounds as Beneficial Phytochemicals in Pomegranate (Punica granatum L.) Peel: a Review. Food Chem. (2018) 261: 75-86.
[4] Akhtar S, Ismail T, Fraternale D, Sestili P. Pomegranate Peel and Peel Extracts: Chemistry and Food Features. Food Chem. (2015) 174: 417-25.
[5] Amri Z, Zaouay F, Lazreg-Aref H, Soltana H, Mneri A, Mars M, Hammami M. Phytochemical Content, Fatty Acids Composition and Antioxidant Potential of Different Pomegranate Parts: Comparison Between Edible and Non Edible Varieties Grown In Tunisia. Int. J. Biol. Macromol. (2017) 104: 274-80.
[6] Heber D, Schulman RN, Seeram NP. (eds.) Pomegranates: Ancient Roots to Modern Medicine. CRC press, New York (2006) 44-46.
[7] Zhao X, Yuan Z, Fang Y, Yin Y, Feng L. Characterization and Evaluation of Major Anthocyanins in Pomegranate (Punica granatum L.) Peel of Different Cultivars and Their Development Phases. Eur. Food Res. Technol. (2013) 236(1): 109-17.
[8] Puneeth H, Chandra S. A Review on Potential Therapeutic Properties of Pomegranate (Punica granatum L.). Plant Sci. Today (2020) 7(1): 9-16.
[9] Vučić V, Grabež M, Trchounian A, Arsić A. Composition and Potential Health Benefits of Pomegranate: a Review. Curr. Pharm. Des. (2019) 25(16): 1817-27.
[10] Pirzadeh M, Caporaso N, Rauf A, Shariati MA, Yessimbekov Z, Khan MU, Imran M, Mubarak MS. Pomegranate as a Source of Bioactive Constituents: a Review on Their Characterization, Properties and Applications. Crit. Rev. Food Sci. Nutr. (2020): 1-18.
[11] Fischer UA, Carle R, Kammerer DR. Identification and Quantification of Phenolic Compounds from Pomegranate (Punica granatum L.) Peel, Mesocarp, Aril and Differently Produced Juices by HPLC-DAD–ESI/MSn. Food Chem. (2011) 127(2): 807-21.
[12] Gavrilova V, Kajdzanoska M, Gjamovski V, Stefova M. Separation, Characterization and Quantification of Phenolic Compounds in Blueberries and Red and Black Currants by HPLC− DAD− ESI-MS n. J. Agric. Food Chem. (2011) 59(8): 4009-18.
[13] Harnly JM, Bhagwat S, Lin L-Z. Profiling Methods for the Determination of Phenolic Compounds in Foods and Dietary Supplements. Anal. Bioanal. Chem. (2007) 389(1): 47-61.
[14] Braca A, Tommasi N, Bari L, Pizza C, Politi M, Morelli I. Antioxidant Principles from Bauhinia T Arapotensis. J. Nat. Prod. (2001) 64(7): 892-5.
[15] Zahin M, Aqil F, Ahmad I. Broad Spectrum Antimutagenic Activity of Antioxidant Active Fraction of Punica granatum L. peel extracts. Mutat. Res. Genet. Toxicol. Environ. Mutagen. (2010) 703(2): 99-107.
[16] Pluskal T, Castillo S, Villar-Briones A, Orešič M. MZmine 2: Modular Framework for Processing, Visualizing, and Analyzing Mass Spectrometry-Based Molecular Profile Data. BMC Bioinformatics (2010) 11(1): 395.
[17] Abid M, Yaich H, Cheikhrouhou S, Khemakhem I, Bouaziz M, Attia H, Ayadi MA. Antioxidant Properties and Phenolic Profile Characterization by LC–MS/MS of Selected Tunisian Pomegranate Peels. J. Food Sci. Technol. (2017) 54(9): 2890-901.
[18] Elfalleh W, Nasri N, Marzougui N, Thabti I, M'rabet A, Yahya Y, Lachiheb B, Guasmi F, Ferchichi A. Physico-Chemical Properties and DPPH-ABTS Scavenging Activity of Some Local Pomegranate (Punica granatum) Ecotypes. Int. J. Food Sci. Nutr. (2009) 60(sup2): 197-210.
[19] Elfalleh W, Tlili N, Nasri N, Yahia Y, Hannachi H, Chaira N, Ying M, Ferchichi A. Antioxidant Capacities of Phenolic Compounds and Tocopherols from Tunisian Pomegranate (Punica granatum) Fruits. J. Food Sci. (2011) 76(5): C707-C13.
[20] Elfalleh W, Hannachi H, Tlili N, Yahia Y, Nasri N, Ferchichi A. Total Phenolic Contents and Antioxidant Activities of Pomegranate Peel, Seed, Leaf and Flower. J. Med. Plants Res. (2012) 6(32): 4724-30.
[21] Mansour E, Ben Khaled A, Lachiheb B, Abid M, Bachar K, Ferchichi A. Phenolic Compounds, Antioxidant, and Antibacterial Activities of Peel Extract from Tunisian Pomegranate. J. Agr. Sci. Tech. (2013) 15: 1393-403.
[22] Ardekani MRS, Hajimahmoodi M, Oveisi MR, Sadeghi N, Jannat B, Ranjbar AM, Gholam N, Moridib T. Comparative Antioxidant Activity and Total Flavonoid Content of Persian Pomegranate (Punica granatum L.) Cultivars. Iran J. Pharm. Res. (2011) 10(3): 519.
[23] Doostan F, Vafafar R, Zakeri-Milani P, Pouri A, Afshar RA, Abbasi MM. Effects of Pomegranate (Punica granatum L.) Seed and Peel Methanolic Extracts on Oxidative Stress and Lipid Profile Changes Induced by Methotrexate in Rats. Adv. Pharm. Bull. (2017) 7(2): 269.
[24] Shishavan N, Abbasi M, Afshar R, Milani P. The Effects of Pomegranate (Punica granatum L.) Peel Methanolic Extract on Methotrexate Induced Changes in Hepatic Antioxidant Enzymes of Rats. Jundishapur J. Nat. Pharm. Prod. (2017) 12(1): e57499.
[25] Arun K, Jayamurthy P, Anusha C, Mahesh S, Nisha P. Studies on Activity Guided Fractionation of Pomegranate Peel Extracts and Its Effect on Antidiabetic and Cardiovascular Protection Properties. J. Food Process Pres. (2017) 41(1):e13108.
[26] Kanatt SR, Chander R, Sharma A. Antioxidant and Antimicrobial Activity of Pomegranate Peel Extract Improves the Shelf Life of Chicken Products. Int. J. Food Sci. (2010) 45(2): 216-22.
[27] Malviya S, Jha A, Hettiarachchy N. Antioxidant and Antibacterial Potential of Pomegranate Peel Extracts. J. Food Sci. Technol. (2014) 51(12): 4132-7.
[28] Masci A, Coccia A, Lendaro E, Mosca L, Paolicelli P, Cesa S. Evaluation of Different Extraction Methods from Pomegranate Whole Fruit or Peels and the Antioxidant and Antiproliferative Activity of the Polyphenolic Fraction. Food Chem. (2016) 202: 59-69.
[29] Pagliarulo C, Vito V, Picariello G, Colicchio R, Pastore G, Salvatore P, Volpe MG. Inhibitory Effect of Pomegranate (Punica granatum L.) Polyphenol Extracts on the Bacterial Growth and Survival of Clinical Isolates of Pathogenic Staphylococcus Aureus and Escherichia Coli. Food Chem. (2016) 190: 824-31.
[30] Ashoush IS, El-Batawy O, El-Shourbagy GA. Antioxidant Activity and Hepatoprotective Effect of Pomegranate Peel and Whey Powders in Rats. Ann. Agric. Sci. (2013) 58(1): 27-32.
[31] Fawole OA, Makunga NP, Opara UL. Antibacterial, Antioxidant and Tyrosinase-Inhibition Activities of Pomegranate Fruit Peel Methanolic Extract. BMC Complement. Altern. Med. (2012) 12(1): 200.
[32] Khalil A, Khan M, Shabbir M, Rahman K. Comparison of Antioxidative Potential and Punicalagin Content of Pomegranate Peels. J. Anim. Plant. Sci. (2017) 27(2): 522-7.
[33] Orak HH, Yagar H, Isbilir SS. Comparison of Antioxidant Activities of Juice, Peel, and Seed of Pomegranate (Punica granatum L.) and Inter-Relationships With Total Phenolic, Tannin, Anthocyanin, and Flavonoid Contents. Food Sci. Biotechnol. (2012) 21(2): 373-87.
[34] Guo C, Yang J, Wei J, Li Y, Xu J, Jiang Y. Antioxidant Activities of Peel, Pulp and Seed Fractions of Common Fruits as Determined by FRAP Assay. Nutr. Res. (2003) 23(12): 1719-26.
[35] Orgil O, Schwartz E, Baruch L, Matityahu I, Mahajna J, Amir R. The Antioxidative and Anti-Proliferative Potential of Non-Edible Organs of the Pomegranate Fruit and Tree. Food Sci. Technol. (2014) 58(2): 571-7.
[36] Pande G, Akoh CC. Antioxidant Capacity and Lipid Characterization of Six Georgia-Grown Pomegranate Cultivars. J. Agric. Food Chem. (2009) 57(20): 9427-36.
[37] Rosas‐Burgos EC, Burgos‐Hernández A, Noguera‐Artiaga L, Kačániová M, Hernández‐García F, Cárdenas‐López JL, Carbonell‐Barrachina Á. Antimicrobial Activity of Pomegranate Peel Extracts as Affected by Cultivar. J. Sci. Food Agric. (2017) 97(3): 802-10.
[38] Çam M, Hışıl Y. Pressurised Water Extraction of Polyphenols from Pomegranate Peels. Food Chem. (2010) 123(3): 878-85.
[39] Dikmen M, Ozturk N, Ozturk Y. The Antioxidant Potency of Punica granatum L. Fruit Peel Reduces Cell Proliferation and Induces Apoptosis on Breast Cancer. J. Med. Food (2011) 14(12): 1638-46.
[40] Stojanović I, Šavikin K, Đedović N, Živković J, Saksida T, Momčilović M, Koprivica I, Vujičić M, Stanisavljević S, Miljković Đ, Menković N. Pomegranate Peel Extract Ameliorates Autoimmunity in Animal Models of Multiple Sclerosis and Type 1 Diabetes. J. Funct. Foods (2017) 35: 522-30.
[41] Morzelle MC, Salgado JM, Telles M, Mourelle D, Bachiega P, Buck HS, Araujo Viel T. Neuroprotective Effects of Pomegranate Peel Extract after Chronic Infusion with Amyloid-Β Peptide in Mice. PloS one. (2016) 11(11).
[42] Singh JP, Kaur A, Shevkani K, Singh N. Composition, Bioactive Compounds and Antioxidant Activity of Common Indian Fruits and Vegetables. J. Food Sci. Technol. (2016) 53(11): 4056-66.
[43] Zarezadeh Mehrizi R, Emam-Djomeh Z, Bagh Khandan MS, Loni E, Akhavan H, Biabani J. Identification and Quantification of Anthocyanins in Pomegranate Peel Extract. J. Food Sci. Technol. 2015;12(49): 31-40.
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