Assessment of the Skin and Heart Tissue Damage Following Inhalation of Carbon Nanotubes in Wistar Rats Using Isolated Mitochondria Carbon nanotubes induced skin and heart tissue damage
Iranian Journal of Pharmaceutical Sciences,
Vol. 17 No. 1 (2021),
15 January 2021
,
Page 69-78
https://doi.org/10.22037/ijps.v17.40300
Abstract
The unique properties of carbon nanotubes (CNTs) have led to their use in various fields. But, the toxicity of CNTs has been reported in biological and environmental systems. The aim of this research is to study the effect of multi-wall carbon nanotubes (MWCNTs) through inhalation chamber on the mitochondrial damage and oxidative stress using the mitochondria obtained from the skin and heart. Rats were exposed to 5 mg/m3 of MWCNTs (10 nm) aerosol for 5 hours /day, 5 days/week for 2 weeks in a whole-body exposure chamber. After 2-weeks exposure, Heart and skin mitochondria were evaluated for evaluation of toxicity parameters. The results showed that nanoparticles significantly decreased mitochondrial succinate dehydrogenase (SDH) activity and increased the level of reactive oxygen species (ROS), collapse in mitochondria membrane potential (MMP), swelling in mitochondria, and cytochrome release. In conclusion, we suggested that 5 mg/m3 of MWCNTs (10 nm) induce ROS mediated cytotoxicity by directly targeting mitochondria in both skin and heart tissue.
- Heart
- Mitochondria
- Multi Wall Carbon Nanotube
- Skin
- Toxicity
How to Cite
References
[2] Luyts, K., et al. Nanoparticles in the lungs of old mice: Pulmonary inflammation and oxidative stress without procoagulant effects. Sci. Total. Environ (2018) 644: 907-915.
[3] Shen, Z., et al. Comparison of cytotoxicity and membrane efflux pump inhibition in HepG2 cells induced by single-walled carbon nanotubes with different length and functional groups. Sci. Rep (2019) 9(1): 7557.
[4] Thakkar, M., S. Mitra, and L. Wei. Effect on Growth, Photosynthesis, and Oxidative Stress of Single Walled Carbon Nanotubes Exposure to Marine Alga Dunaliella tertiolecta. J. Nanomater (2016) 2016.
[5] Drobne, D. Nanotoxicology for safe and sustainable nanotechnology. Arh. Hig. Rada. Toksikol (2007) 58(4): 471-8.
[6] Umeda, Y. et al. Two-week Toxicity of Multi-walled Carbon Nanotubes by Whole-body Inhalation Exposure in Rats. J. Toxicol. Pathol (2013) 26(2): 131-40.
[7] Johnston, H.J., et al. A critical review of the biological mechanisms underlying the in vivo and in vitro toxicity of carbon nanotubes: The contribution of physico-chemical characteristics. Nanotoxicology (2010) 4(2): 207-46.
[8] Srivastava, S. Sorption of divalent metal ions from aqueous solution by oxidized carbon nanotubes and nanocages: A review. Adv. Mater. Lett (2013) 4(1): 2-8.
[9] Girardello, R., et al., Cellular responses induced by multi-walled carbon nanotubes: in vivo and in vitro studies on the medicinal leech macrophages. Sci Rep, 2017. 7(1): p. 8871.
[10] Icoglu Aksakal, F., A. Ciltas, and N. Simsek Ozek. A holistic study on potential toxic effects of carboxylated multi-walled carbon nanotubes (MWCNTs-COOH) on zebrafish (Danio rerio) embryos/larvae. Chemosphere (2019) 225: 820-828.
[11] Rong, H., et al. Carboxylated multi-walled carbon nanotubes exacerbated oxidative damage in roots of Vicia faba L. seedlings under combined stress of lead and cadmium. Ecotoxicol. Environ. Saf (2018) 161: 616-623.
[12] Alarifi, S. and D. Ali. Mechanisms of Multi-walled Carbon Nanotubes-Induced Oxidative Stress and Genotoxicity in Mouse Fibroblast Cells. Int. J. Toxicol (2015) 34(3): 258-65.
[13] Lee, J.W., et al. Multiwall Carbon Nanotube-Induced Apoptosis and Antioxidant Gene Expression in the Gills, Liver, and Intestine of Oryzias latipes. Biomed. Res. Int (2015) 2015: 485343.
[14] Liu, X., et al. Antioxidant deactivation on graphenic nanocarbon surfaces. Small (2011) 7(19): 2775-85.
[15] Kim, J.S., K.S. Song, and I.J. Yu. Multiwall Carbon Nanotube-Induced DNA Damage and Cytotoxicity in Male Human Peripheral Blood Lymphocytes. Int. J. Toxicol (2016) 35(1): 27-37.
[16] Nogueira, D.R., C.M. Rolim, and A.A. Farooqi. Nanoparticle induced oxidative stress in cancer cells: adding new pieces to an incomplete jigsaw puzzle. Asian. Pac. J. Cancer Prev (2014) 15(12): 4739-43.
[17] Li, B., et al. Single-walled carbon nanohorn aggregates promotes mitochondrial dysfunction-induced apoptosis in hepatoblastoma cells by targeting SIRT3. Int. J. Oncol (2018) 53(3): 1129-1137.
[18] Rezaei, M., et al., A comparison of toxicity mechanisms of dust storm particles collected in the southwest of Iran on lung and skin using isolated mitochondria. Toxicol. Environ. Chem (2014) 96(5): 814-830.
[19] Salimi, A., et al. Toxicity of macrolide antibiotics on isolated heart mitochondria: a justification for their cardiotoxic adverse effect. Xenobiotica (2016) 46(1): 82-93.
[20] Zhao, Y., et al. Vanadium compounds induced mitochondria permeability transition pore (PTP) opening related to oxidative stress. J. Inorg. Biochem (2010) 104(4): 371-8.
[21] Arast, Y. and J. Pourahmad. Selective Toxicity of Standardized Extracts of Persian Gulf Sponge (Irciniamutans) on Skin Cells and Mitochondria isolated from Melanoma induced mouse. Int. Pharm. Acta (2019) 2(1): 2-5: 1-12.
[22] Seydi, E., et al. Hexavalent Chromium Induced Oxidative Stress and Toxicity on isolated human lymphocytes. Int. Pharm. Acta (2020) 3(1): e1.
[23] Francis, A.P. and T. Devasena. Toxicity of carbon nanotubes: A review. Toxicol. Ind. Health (2018) 34(3): 200-210.
[24] Tsukahara, T., Y. Matsuda, and H. Haniu. The role of autophagy as a mechanism of toxicity induced by multi-walled carbon nanotubes in human lung cells. Int. J. Mol. Sci (2014) 16(1): 40-8.
[25] Coccini, T., L. Manzo, and E. Roda. Safety evaluation of engineered nanomaterials for health risk assessment: an experimental tiered testing approach using pristine and functionalized carbon nanotubes. ISRN. Toxicol (2013) 2013: 825427.
[26] Geiser, M., et al. Evaluating Adverse Effects of Inhaled Nanoparticles by Realistic In Vitro Technology. Nanomaterials (Basel) (2017) 7(2).
[27] Gornati, R., et al. In vivo and in vitro models for nanotoxicology testing. Nanotoxicology: From In Vivo and In Vitro Models to Health Risks; Sahu, SC, Casciano, D., Eds, 2009: 279-302.
[28] Visalli, G., et al. Mitochondrial Impairment Induced by Sub-Chronic Exposure to Multi-Walled Carbon Nanotubes. Int. J. Environ. Res. Public. Health (2019) 16(5).
[29] Moller, P., et al. Role of oxidative stress in carbon nanotube-generated health effects. Arch. Toxicol (2014) 88(11): 1939-64.
[30] Lotfipanah, S., M. Zeinali, and P. Yaghmaei. Induction of caspase-2 gene expression in carboxyl-functionalized carbon nanotube-treated human T-cell leukemia (Jurkat) cell line. Drug. Chem. Toxicol (2019): 1-6.
[31] Chen, Z., et al. Anagliptin protects neuronal cells against endogenous amyloid beta (Abeta)-induced cytotoxicity and apoptosis. Artif . Cells. Nanomed. Biotechnol (2019) 47(1): 2213-2220.
[32] Khosravi, Y., et al. Inhalation exposure of nano diamond induced oxidative stress in lung, heart and brain. Xenobiotica (2018) 48(8): 860-866.
[33] Tripathi, A., et al. Di-(2-ethylhexyl) phthalate (DEHP) inhibits steroidogenesis and induces mitochondria-ROS mediated apoptosis in rat ovarian granulosa cells. Toxicol. Res (Camb) (2019) 8(3): 381-394.
[34] Liu, K., et al. The role of cytochrome c on apoptosis induced by Anagrapha falcifera multiple nuclear polyhedrosis virus in insect Spodoptera litura cells. PLoS. One (2012) 7(8): e40877.
[35] Peruzzo, R. and I. Szabo. Contribution of Mitochondrial Ion Channels to Chemo-Resistance in Cancer Cells. Cancers (Basel) (2019) 11(6).
[36] Zhao, X., et al. Zinc oxide nanoparticles induce oxidative DNA damage and ROS-triggered mitochondria-mediated apoptosis in zebrafish embryos. Aquat. Toxicol (2016) 180: 56-70.
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