Potency of Mangosteen Pericarp Extract to Inhibit 38-kDa and Ag85 Protein Secretion by Mycobacterium tuberculosis H37Rv Mangosteen pericarp inhibit Tuberculosis Protein secretion
Iranian Journal of Pharmaceutical Sciences,
Vol. 16 No. 2 (2020),
1 April 2020
,
Page 19-26
https://doi.org/10.22037/ijps.v16.40421
Abstract
Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. The α-mangostin in mangosteen pericarp extract can inhibit M. tuberculosis growth. This study examined the potency of α-mangostin in mangosteen pericarp extract to inhibit 38-kDa and Ag85 protein secretion from M. tuberculosis H37Rv. The samples used in this study were divided into three independent variable groups (3.125 μg/ml; 6.25 μg/ml; and 12.5 μg/ml α-mangostin in the mangosteen pericarp extract), a positive control group (rifampin), a negative control group (M. tuberculosis H37Rv), and the Garcia® group (trademark of the mangosteen peel extract capsule). HPLC-MS/MS detected that the mangosteen pericarp extract contained 5,984.55 μg/g α-
mangostin. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and dot blot analysis were conducted to analyze the profile and specificity of both proteins. This study revealed that 6.25 μg/ml of mangosteen pericarp extract had the greatest potency for inhibiting 38-kDa protein secretion from M. tuberculosis H37Rv, and 12.5 μg/ml mangosteen pericarp extract had greater potency for inhibiting Ag85 protein secretion than did the other doses. Thus, α-mangosteen from mangosteen pericarp extract can inhibit Ag38 and Ag85 secretion.
- α-mangostin
- Ag38 kDa
- Ag85
- Mycobacterium tuberculosis
How to Cite
References
[2] Wang W, Liao Y, Huang X, Tang C, Cai P. A novel xanthone dimer derivative with antibacterial activity isolated from the bark of Garcinia mangostana.Nat. Prod. Res. (2018) 32: 1769-1774.
[3] Ovalle-Magallanes B, Eugenio-Perez D, Pedraza-Chaverri J. Medicinal properties of mangosteen (Garcinia mangostana L.): A comprehensive update.Food and Chem. Toxicol. (2017) 109: 102-122.
[4] Huygen K. The Immunodominant T-Cell Epitopes of the Mycolyl-Transferases of the Antigen 85 Complex of M. tuberculosis. Front. Immunol. (2014) 5 (321): 1-11.
[5] Maliana Y, Khotimah S, Diba F. Aktivitas Antibakteri Kulit Garcinia mangostana Linn. terhadap Pertumbuhan Flavobacterium dan Enterobacter coptotermes curvinathus Holmgren (In Indonesia). J.Protobiont (2013) 2 (1): 7-11.
[5] Bundeesomchok K, Filly A, Rakotomanomana N,Panichayupakaranant P, Chemat F. Extraction of α-mangostin from Garcinia mangostana L. using alternative solvents: Computational predictive and experimental studies. Food Sci. and Tech. (2016) 65:296-303.
[6] Tiwari D, Tiwari RP, Chandra R, Bisen PS, Haque S. Efficient ELISA for Diagnosis of Active Tuberculosis Employing a Cocktail of Secretory of Mycobacterium tuberculosis. Folla Biol. (Praha) (2014) 60: 10-20.
[7] Nataraj V, Varela C, Javid A, Singh A, Besra GS, Bhatt A. Mycolic acids: deciphering and targeting the Achilles' heel of the tubercle bacillus. Mol. Microbiol.(2015) 98: 7-16.
[8] Sieniawska E, Swatko-Ossor M, Sawicki R, Skalicka-Woźniak K, Ginalska G. Natural Terpenes Influence the Activity of Antibiotics against Isolated Mycobacterium tuberculosis. Medical Prin. and Pract.(2017) 26: 108-112.
[9] Beer LA, Speicher DW. Protein Detection in Gels Using Fixation. Curr. Protoc. in Protein Sci. (2018) 91: 10501–10520.
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