Investigating the Antimicrobial Efficacy of Liposomal Vancomycin in Gram-positive and Gram-negative bacteria- A Preliminary Mechanistic Study Antimicrobial Effects of Liposomal Vancomycin
Iranian Journal of Pharmaceutical Sciences,
Vol. 14 No. 3 (2018),
1 Tir 2018
,
Page 13-24
https://doi.org/10.22037/ijps.v14.40635
Abstract
Outer membrane of Gram-negative bacteria is a permeability barrier to many antibacterial agents, including the glycopeptide antibiotics such as vancomycin hydrochloride and as a result these antibiotics are ineffective against Gram negative bacteria. Different strategies have been described to overcome such limitation, including application of nanoparticles, as was shown in our previous studies for polymeric nanoparticles. On the other hand, some nanoparticles have the ability to reduce the permeation of drugs through biological barriers. Therefore, in this investigation, the effects of fusogenic liposomes, which are expected to interact well with biological barriers, toward antimicrobial effects of vancomycin in different bacteria, are investigated.Vancomycin-loaded liposomes were prepared by lipid film hydration method from a phospholipid mixture composed of either DPPC: DOPE: Chol or DPPC: DOPE: CHEMS, both in 1: 0.5: 1 molar ratios. Obtained liposomes were then assessed in regard to their antibacterial properties using broth microdilution method. Liposomes were prepared by lipid-film hydration followed by extrusion and probe sonication for size reduction. Encapsulation efficiency for large hydrophilic vancomycin in liposomes was found to be in the range of 0.1 to 9 % for different formulations. Probe sonicated liposomes showed smaller size and were more stable than those prepared by extrusion. Antimicrobial results showed that encapsulation of vancomycin in liposomes decreased antibacterial efficacy of vancomycin and caused MIC increments, compared to those of free vancomycin. This might indicate negligible release of this large and charged molecule from liposomes into the bacterial preplasmic space (retention of vancomycin inside liposomal cavity or lipid-drug complexation) accompanied by inability of liposomes to permeate the bacterial barrier. Further investigations are needed to explain the interaction of liposomes with bacterial membranes.
- Vancomycin
- Fusogenic liposomes
- Antibacterial efficacy
- Bacterial resistance
- Outer membrane
- Retardation
How to Cite
References
[2] Center for Disease Control. Antibiotic resistance threats in the United States. Washington (2013):100- 114
[3] O’Neill J. Antimicrobial Resistance : Tackling a crisis for the health and wealth of nations. Rev. Antimicrob. Resist. (2014):1–16.
[4] World Health Organization. Antimicrobial resistance: global report on surveillance. 2014. Available from URL: http://www.who.int/drugresistance/documents/surveillancereport/en/
[5] McGowan JE. Economic impact of antimicrobial resistance. Emerg. Infect. Dis. (2001) 2:286–92.
[6] Sheldrick, GM, Jones PG, Kennard O, Williams DH and Smith GA. Structure of vancomycin and its complex with acetyl-D-alanyl-D-alanine. Nature. (1978) 5642: 223-225.
[7] Reynolds PE. Structure, biochemistry and mechanism of action of glycopeptide antibiotics. Eur. J. Clin. Microbiol. Infect. Dis. (1989) 11: 943-950.
[8] Williams DH. The glycopeptide story how to kill the deadly 'superbugs'. Nat Prod Rep. (1996) 13(6): 469-477.
[9] Silhavy TJ, Kahne D and Walker S. The bacterial cell envelope. Cold Spring Harb. Perspect. Biol. (2010)2: a000414.
[10] Delcour AH. Outer membrane permeability and antibiotic resistance. Biochim. Biophys. Acta. (2009) 1794(5): 808-816.
[11] Zgurskaya HI, Löpez CA and Gnanakaran S. Permeability barrier of gram-negative cell envelopes and approaches to bypass it. ACS infectious diseases. (2015) 1(11): 512-522.
[12] Watanakunakorn, C. Mode of action and in-vitro activity of vancomycin. J. Antimicrob. Chemother. (1984) 14 Suppl D: 7-18.
[13] Duzgune S and Nir S. Mechanisms and kinetics of liposome-cell interactions. Adv. Drug Deliv. Rev. (1999) 40(1-2): 3-18.
[14] Bakker JA, Woudenberg JM, Ten MT, Stearne-Cullen LET, Woodle MC. Efficacy of gentamicin or ceftazidime entrapped in liposomes with prolonged blood circulation and enhanced localization in klebsiella pneumoniae-infected lung tissue. J. Infect. Dis. (1995);171(4):938–47.
[15] Mugabe C, Halwani M, Azghani A. Mechanism of enhanced activity of liposome-entrapped aminoglycosides against resistant strains of Pseudomonas aeruginosa. Antimicrob. Agents and Chemother. (2008) 50:2016-22.
[16] Ghanbarzadeh, S, Valizadeh H and Zakeri-Milani P. The effects of lyophilization on the physicochemical stability of sirolimus liposomes. Advanced Pharmaceutical Bulletin. (2013) 3(1): 25-29.
[17] Stewart JCM. Colorimetric determination of phospholipids with ammonium ferrothiocyanate. Analytical Biochemistry. (1980) 104(1): 10-14.
[18] Serri A, Moghimi HR, Mahboubi A, Zarghi A. Stability-indicating HPLC method for determination of vancomycin hydrochloride in the pharmaceutical dosage forms. Acta Pol. Pharm. Drug Res. (2017) 74(1):73–9.
[19] Ghaffari A, Manafi A, Moghimi HR. Clindamycin phosphate absorption from nanoliposomal formulations through third-degree burn eschar. World J Plast Surg. (2015) 4(2):145–52.
[20] Mahboubi A, Asgarpanah J, Sadaghiyani PN and Faizi M. Total phenolic and flavonoid content and antibacterial activity of Punica granatum L. var. pleniflora flowers (Golnar) against bacterial strains causing foodborne diseases. BMC Complementary and Alternative Medicine. (2015) 15: 366.
[21] Maulucci G, Spirito M, Arcovito G, Boffi F, Castellano AC and Briganti G. Particle size distribution in DMPC vesicles solutions undergoing different sonication times. Biophys. J. (2005) 88(5): 3545-3550.
[22] Lapinski, MM, Castro-Forero A, Greiner AJ, Ofoli RY and Blanchard GJ. Comparison of liposomes formed by sonication and extrusion: rotational and translational diffusion of an embedded chromophore. Langmuir : the ACS journal of surfaces and colloids. (2007) 23(23).
[23] Cho NJ, Hwang LY, Solandt JJR and Frank CW. Comparison of extruded and sonicated vesicles for planar bilayer self-assembly. Materials. (2013) 6(8): 3294-3308.
[24] Eloy JO, ClarodeSouza M, Petrilli R, Barcellos JP, Lee RJ and Marchetti JM. Liposomes as carriers of hydrophilic small molecule drugs: strategies to enhance encapsulation and delivery. Colloids Surf Biointerfaces (2014) 123: 345-363.
[25] Xiao C, Qi X, Maitani Y, Nagai T. Sustained release of cisplatin from multivesicular liposomes: Potentiation of antitumor efficacy against S180 murine carcinoma. J. Pharm. Sci. (2004) 93(7):1718–24.
[26] Tseng LP, Liang HJ, Chung TW, Huang YY, Liu DZ. Liposomes incorporated with cholesterol for drug release triggered by magnetic field. J. Med. Biol. Eng. (2007) 27(1):29–34.
[27] Haeri A, Sadeghian S, Rabbani S, Anvari MS, Erfan M, Dadashzadeh S. PEGylated estradiol benzoate liposomes as a potential local vascular delivery system for treatment of restenosis. J Microencapsul (2012) 29(1):83–94.
[28] Yang Z, Lu A, Wong BCK, Chen X, Bian Z, Zhao Z, et al. Effect of liposomes on the absorption of water-soluble active pharmaceutical ingredients via oral administration. Curr. Pharm. Des. (2013) 19(37):6647–54.
[29] Nounou MM, El-Khordagui LK, Khalafallah N a, Khalil S. In vitro release of hydrophilic and hydrophobic drugs from liposomal dispersions and gels. Acta Pharm. (2006) 56(3):311–24.
[30] Ghaffari A, Manafi A, Moghimi HR. Clindamycin phosphate absorption from nanoliposomal formulations through third-degree burn eschar. World J. Plastic. Surg. (2015) 4(2):145+152.
[31] Hafez IM, Pieter R. Cullis PR. Cholesteryl hemisuccinate exhibits pH sensitive polymorphic phase behavior. Biochimica. et. Biophysica. Acta. (2000):107-114.
[32] Serri A, Mahboubi A, Zarghi A, Moghimi HR. PAMAM-dendrimer enhanced antibacterial effect of vancomycin hydrochloride against gram-negative bacteria. J. Pharm. Pharm. Sci. (2019) 22: 10-21
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