Histopathological and Biomedical Parameters Determination in the Protective Effect of Hydroalcoholic Extract of Allium Jesdianum on Hepatotoxicity Induced by Bromobenzene Effect of Allium Jesdianum Extract on Bromobenzene Induced Hepatotoxicity
Iranian Journal of Pharmaceutical Sciences,
Vol. 14 Núm. 2 (2018),
1 April 2018
,
Página 15-24
https://doi.org/10.22037/ijps.v14.40656
Resumen
Allium Jesdianum (AJ) is the native plant mostly grown in Middle East region that has the excellent pharmacological properties. In this study, we evaluated the hepatoprotective effect of hydroalcoholic extract of AJ on liver injury induced by Bromobenzene (BB) in male mice. Animals were randomly divided into five groups, control group received normal saline plus olive oil, groups 2-4 received (500, 1000 and 2000 mg/kg) AJ extract plus BB for 5 days and 5th group received BB (460 mg/kg). On the fifth day all groups received hexobarbital sodium (25 mg/kg, i.p). It should be noted that sleeping time of the all mice were recorded. After 24 hours the mice were sacrificed. By serum and tissue biomarkers such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), reduced glutathione (GSH), malondialdehyde (MDA), and histopathological studies were measured. The results showed that BB significantly increased sleeping time, ALT, AST, and MDA levels besides and decreased GSH level compared with the control group. AJ extract at doses1000and 2000 mg/kg showed a significant alter in all studied endpoints and dose 2000 mg/kg showed a marked improvement in histopathological examination. The present finding indicated that administration of the hydroalcoholic extract of AJ could prevent hepatotoxicity induced by BB via improvement serum and tissue parameters and histopathological alterations in liver tissue.
- Bromobenzene
- Allium Jesdianum
- Hepatoprotective
- Antioxidants
- Glutathione
- Malondialdehyde
Cómo citar
Citas
[2] Bantel H, Schulze-Osthoff K. Mechanisms of cell death in acute liver failure. Front Physiol. 2012;3(79):1-8.
[3]Kalantari H, Kooshapur H, Rezaii F, Ranjbari N, Moosavi M. Study of the protective effect of Raphanus sativus (radish) seed in liver toxicity induced by carbon tetrachloride in mice. Jundishapur J Nat Pharm Prod. 2009;4(1):24-31.
[4] Brautbar N, Williams J. Industrial solvents and liver toxicity: risk assessment, risk factors and mechanisms. Int J Hyg Environ Health. 2002;205(6):479-91.
[5] El-Sharaky A, Newairy A, Kamel M, Eweda S. Protective effect of ginger extract against bromobenzene-induced hepatotoxicity in male rats. Food Chem Toxicol. 2009;47(7):1584-90.
[6]Vedi M, Sabina EP. Assessment of hepatoprotective and nephroprotective potential of withaferin A on bromobenzene-induced injury in Swiss albino mice: possible involvement of mitochondrial dysfunction and inflammation. Cell Biol. Toxicol. 2016;32(5):373-90.
[7] Sabina EP, Martin SJ, Vedi M, Lavinya BU, Benny B, ST AS, et al. Protective role of food supplement Spirulina fusiformis in chemical induced hepatotoxicity: a bromobenzene model in rats. Int. j. pharm. Res. 2014;4(1):04-9.
[8]Cheol Park J, Chul Kim S, Moon Hur J, Choi SH, Yeon Lee K, Won Choi J. Anti-hepatotoxic effects of Rosa rugosa root and its compound, rosamultin, in rats intoxicated with bromobenzene. J Med Food. 2004;7(4):436-41.
[9]Vedi M, Rasool M, Sabina EP. Amelioration of bromobenzene hepatotoxicity by Withania somnifera pretreatment: role of mitochondrial oxidative stress. Toxicol Rep. 2014;1:629-38.
[10] Hamed M, Ali S. Effects of black seed oil on resolution of hepato-renal toxicity induced by bromobenzene in rats. Eur Rev Med Pharmacol Sci. 2013;17(5):569-81.
[11]Yoshioka H, Fukaya S, Fukuishi N, Nagatsu A, Nonogaki T, Onosaka S. Bromobenzene-induced lethal toxicity in mouse is prevented by pretreatment with zinc sulfate. Chem. Biol. Interact. 2016;254:117-23.
[12] Mahima V, Martin S, Lavinya B, Yashodhara B, Monisha S, Shreni T, et al. Ameliorative effects of Spirulina fusiformis against bromobenzene induced nephrotoxicity in rats: A Biochemical and Histoarchitectural study. Asian J. Biochem. 2014;9(1):57-64.
[13] Lorigooini Z, Ayatollahi SA, Amidi S, Kobarfard F. Evaluation of anti-platelet aggregation effect of some Allium species. iranJ Pharm Res: IJPR. 2015;14(4):1225.
[14] Kshirsagar A, Mohite R, Aggrawal A, Suralkar U. Hepato-protective medicinal plants of Ayurveda: a review. Asian J Pharm Clin Res. 2011;4(3):1-8.
[15]Rios J, Recio M. Medicinal plants and antimicrobial activity. J Ethnopharmacol. 2005;100(1):80-4.
[16]Arif T, Bhosale J, Kumar N, Mandal T, Bendre R, Lavekar G, et al. Natural products–antifungal agents derived from plants. J Asian Nat Prod Res. 2009;11(7):621-38.
[17] Tepe B, Sokmen M, Akpulat HA, Sokmen A. In vitro antioxidant activities of the methanol extracts of five Allium species from Turkey. Food Chem. 2005;92(1):89-92.
[18] Vahdani R, Mehrabi S, Malekzadeh J, Jannesar R, Sadeghi H, Shafaeifar A. Effect of hydrophilic extract of Allium jesdianum on ethylene glycol-induced renal stone in male wistar rats. Armaghane danesh. 2012;16(6):566-77.
[19]Gholami A, Arabestani MR, Ahmadi M. Evaluation of antibacterial activity of aqueous and methanol extracts of Allium Jesdianum plant on a number of pathogenic bacteria resistant to antibiotics. PSJ. 2016;14(4):18-26.
[20] Pradeep K, Mohan C, Anand K, Karthikeyan S. Effect of pretreatment of Cassia fistula Linn. leaf extract against subacute CCl 4 induced hepatotoxicity in rats. Indian J Exp Biol. 2005;43(6):526.
[21] Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976;72(1-2):248-54.
[22]Dash DK, Yeligar VC, Nayak SS, Ghosh T, Rajalingam R, Sengupta P, et al. Evaluation of hepatoprotective and antioxidant activity of Ichnocarpus frutescens (Linn.) R. Br. on paracetamol-induced hepatotoxicity in rats. Trop J Pharm Res. 2007;6(3):755-65.
[23] Soudani N, Amara IB, Sefi M, Boudawara T, Zeghal N. Effects of selenium on chromium (VI)-induced hepatotoxicity in adult rats. Exp Toxicol Pathol. 2011;63(6):541-8.
[24] Forouzandeh H, Azemi ME, Rashidi I, Goudarzi M, Kalantari H. Study of the protective effect of Teucrium polium L. extract on acetaminophen-induced hepatotoxicity in mice. Iran J Pharm Res.2013;12(1):123-9.
[25] Rajesh M, Latha M. Protective activity of Glycyrrhiza glabra Linn. on carbon tetrachloride-induced peroxidative damageIndian J. Pharmacol. 2004;36(5):284.
[26] Noguchi T, Fong K-L, Lai EK, Alexander SS, King MM, Olson L, et al. Specificity of a phenobarbital-induced cytochrome P-450 for metabolism of carbon tetrachloride to the trichloromethyl radical. Biochem Pharmacol. 1982;31(5):615-24.
[27] Mansour DF, EL-Denshary ES, Bahgat AK, Nada SA. Hep atop rotective Effect of Alpha Lipoic Acid Against Bromobenzene-Induced Liver Damage in Rats. Med J Cairo Univ. 2009;77(4).
[28] Park J-C, Hur J-M, Hwang Y-H, Choi M-R, Kim S-N, Choi J-W. Hepatoprotective activities of Rosa davurica root extract in rats intoxicated with bromobenzene. J. Life Sci. 2003;13(2):230-5.
[29] Kalantari H, Nazari Z, Keliddar A, Foruozandeh H, Kalantar M. Study of the Protective Effect of Livergol against Liver Toxicity Caused by Bromobenzene in Mice. IJPS. 2015;10(2):11-20
[30] Gilani A-UH, Janbaz KH. Preventive and curative effects of Artemisia absinthium on acetaminophen and CCl4-induced hepatotoxicity. Gen Pharmacol-Vasc S. 1995;26(2):309-15.
[31] Messarah M, Klibet F, Boumendjel A, Abdennour C, Bouzerna N, Boulakoud MS, et al. Hepatoprotective role and antioxidant capacity of selenium on arsenic-induced liver injury in rats. Exp Toxicol Pathol. 2012;64(3):167-74.
[32]Gopi S, Setty OH. Protective effect of Phyllanthus fraternus against bromobenzene induced mitochondrial dysfunction in rat liver mitochondria. Food Chem Toxicol. 2010;48(8):2170-5.
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