Phytochemical Screening, Phenolic Content and Antioxidant Activity of Citrus aurantifolia L. Leaves Grown in Two Regions of Oman Antioxidant activity and quantification of phenolics in Omani lime
Iranian Journal of Pharmaceutical Sciences,
Vol. 14 No. 1 (2018),
15 Dey 2018
,
Page 27-34
https://doi.org/10.22037/ijps.v14.40669
Abstract
Lime or Citrus aurantifolia L., is a small citrus fruit that belongs to the family Rutaceae. The fruit and leaves of C. aurantifolia have been used traditionally for weight loss, skin care, relief from constipation and treatment of scurvy, etc. The purpose of this study was to determine the flavonoid, total phenolic content, and investigation of anti-oxidant potential of Citrus aurantifolia leaves grown in Oman. Lime leaves were collected from Batinah (Nakhal) and Ad-dakhiliya (Nizwa) region of Oman. The leaves were dried, powdered, and extracted with ethanol using a hot extraction method. Standard chemical tests were used to check the presence of secondary plant metabolites in the lime leaves extract. Quantitative analysis of the phenolics and flavonoids were done by colorimeteric methods. The antioxidant activity was assessed by DPPH and phosphomolybdenum in vitro assay methods respectively. Chemical analysis of the lime leaves extract showed the presence of major secondary plant metabolites. The obtained results revealed variation in the content of flavonoid (41.38-64.2 μg of QE/mg of dry extract) and phenolic compounds (96.55-322.57 μg of GAE/mg of dry extract). Both the lime leaves extracts from Nakhal and Nizwa showed concentration dependent moderate anti-oxidant activity (11.79-56.89 and 10.11-51.91%). Lime leaves from Batinah region also exhibited better total antioxidant capacity calculated with reference to the standard gallic acid. The aerial part of C. aurantifolia seems to be a rich source of flavonoids. Further studies are recommended to isolate and quantify the pure phytoconstituents from lime leaves grown in the Batinah (Nakhal) region which might serve as natural antioxidants in food and drug industry.
- Antixidants
- Citrus aurantifolia
- DPPH
- Flavonoids
- Omani lime
- Total phenol
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References
[2] Petrovska BA. Historical Review of Medicinal Plants’ Usage. Pharmacog Rev (2012) 6(11): 1–5.
[3] Su HJ, Hogenhout SA, Al-Sadi AM, Kuo CH. Complete Chloroplast Genome Sequence of Omani Lime (Citrus aurantiifolia) and Comparative Analysis within the Rosids. PLoS ONE (2014) 9(11); e113049.
[4] http://www.naturallifeenergy.com/lemon-vs-lime/ [Aniys A, Lemon vs Lime – Differences, Benefits, and Effects on Alkalinity. Natural Life Energy 2015.]
[5] Dongmo PMJ, Tatsadjieu LN, Sonwa ET, Kuate J, Zollo PHA, Menut C. Essential Oils of Citrus aurantifolia from Cameroon and their Antifungal Activity Against Phaeoramularia angolensis, Afr J Agri Res (2009) 4(4): 354-358.
[6] Uttara B, Singh AV, Zamboni P, Mahajan RT. Oxidative Stress and Neurodegenerative Diseases: A Review of Upstream and Downstream Antioxidant Therapeutic Options. Curr Neuropharmacol (2009) 7(1): 65–74.
[7] Al-Brashdi AS, Al-Ariymi H, Al Hashmi M, Khan SA. Phytochemical Screening, Total Phenolic Content and Evaluation of Antioxidant Potential of Aerial Parts of Haplophyllum tuberculatum (Forssk) A. Juss. J Costal Life Med (2016) 4(4): 315-319.
[8] Cai Y, Luo Q, Sun M, Corke H. Antioxidant Activity and Phenolic Compounds of 112 Chinese Medicinal Plants Associated with Anticancer. Life Sci (2004) 74: 2157-2184.
[9] Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem (1999) 64:555-559.
[10] Alhakmani F, Kumar S, Khan SA. Estimation of Total Phenolic Content, In-Vitro Antioxidant and Anti-Inflammatory Activity of Flowers of Moringa oleifera. Asian Pac J Trop Biomed (2013) 3(8): 623-627.
[11] Prieto P, Pineda M, Aguilar M. Spectrophotometric Quantitation of Antioxidant Capacity Through the Formation of Phosphomolybdenum Complex: Specific Application to Determination of Vitamin E. Analytical Biochem (1999) 269: 337–341.
[12] Al Harthi SS, Mavazh A, Al Mahroqi H, Khan. Quantification of Phenolic Compounds, Evaluation of Physicochemical Properties and Antioxidant Activity of Four Date (Phoenix dactylifera L.) Varieties
Cultivated In Oman- A Comparative Study. J Taibah Uni Med Sci (2015) 10(3): 346-352.
[13] Barile E, Bonanomi G, Antignani V, Zolfaghari B, Sajjadi E, Scala F, Lanzotti V. Phytochemical Screening and Antimicrobial Assessment of Abutilon mauritianum, Bacopa monifera and Datura stramonium. Phytochem (2007) 68: 596–603.
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