Comparison between Alpha and Calendula for Healing of Third-Degree Burn in Rats Burn healing in rat by Alpha and Calendula
Iranian Journal of Pharmaceutical Sciences,
Vol. 13 No. 2 (2017),
1 April 2017
,
Page 51-60
https://doi.org/10.22037/ijps.v13.40696
Abstract
In this study, we compared the effects of Calendula and Alpha ointment in the treatment of burn wounds and also compared its results with silver sulfadiazine (SSD). Seventy-five male Sprague-Dawley rats were divided into five groups, and similar burn ulcers were produced on anterior surface of thigh of rats. In the first group of rats no treatment was applied, base gel was applied topically to group II, in groups III-V, Alpha, SSD, and Calendula preparations were applied, respectively. Wound healing, contraction, and histopathological evaluation were evaluated at the end of 7, 14, and 21 days. Alpha ointment was equally effective as Calendula gel, and had better efficacy compared to SSD for all markers of wound healing at days of 7, 14, and 21.Alpha and Calendula preparations are less expensive drugs and significantly improve the quality of wound healing and scar formation and are more appropriate treatment choices than SSD. Therefore, we recommended them as alternative to SSD, especially in patients with low economical backgrounds or in those who show adverse reactions to SSD.
Keywords:
- Alpha
- Calendula
- Silver sulfadiazine
- wound healing
- burn
- Rat
How to Cite
Nader Tanideh, Milad Yavari, Anahita Seddighi, Omid Koohi Hosseinabadi, Melika Farshad, Maral Mokhtari, Babak Shirazi Yeganeh, Akram Jamshidzadeh, Sajad Daneshi, & Behrouz Nikahval. (2017). Comparison between Alpha and Calendula for Healing of Third-Degree Burn in Rats: Burn healing in rat by Alpha and Calendula. Iranian Journal of Pharmaceutical Sciences, 13(2), 51–60. https://doi.org/10.22037/ijps.v13.40696
References
[1] Edraki M, Akbarzadeh A, Hosseinzadeh M, Tanideh N, Salehi A, Koohi-Hosseinabadi O. Healing effect of sea buckthorn, olive oil, and their mixture on full-thickness burn wounds. Adv Skin Wound Care (2014) 27(7): 317-23.
[2] Tanideh N, Rokhsari P, Mehrabani D, Mohammadi Samani S, Sabet Sarvestani F, Ashraf MJ, Koohi Hosseinabadi O, Shamsian S, Ahmadi N. The healing effect of licorice on Pseudomonas aeruginosa infected burn wounds in experimental rat model. WJPS (2014) 3(2): 99-106.
[3] Sai KP, Babu M. Traditional medicine and practices in burn care: need for newer scientific perspectives. Burns (1998) 24(5): 387-8.
[4] Ashkani-Esfahani S, Imanieh MH, Khoshneviszadeh M, Meshksar A, Noorafshan A, Geramizadeh B, Ebrahimi S, Handjani F, Tanideh N. The healing effect of arnebia euchroma in second degree burn wounds in rat as an animal model. IRAN RED CREACENT MED J (2012) 2012(2): 70-4.
[5] Albertyn R, Berg A, Numanoglu A, Rode H. Traditional burn care in sub-Saharan Africa: a long history with wide acceptance. Burns (2015) 41(2): 203-11.
[6] Stipcevic T, Piljac A, Piljac G. Enhanced healing of full-thickness burn wounds using di-rhamnolipid. Burns (2006) 32(1): 24-34.
[7] Mohsenikia M, Nuraei H, Karimi F, Jamalnia N, Esfahani SA, Rafiee S, Azizian Z, Moradi A. Comparing Effects of Arnebia euchroma and Alpha Ointment on Wound Healing Process. Thrita (2015) 4(1).
[8] Hosseini SV, Tanideh N, Kohanteb J, Ghodrati Z, Mehrabani D, Yarmohammadi H. Comparison between Alpha and silver sulfadiazine ointments in treatment of Pseudomonas infections in 3rd degree burns. Int J Surg (2007) 5(1): 23-6.
[9] Nayak BS, Isitor G, Davis E, Pillai G. The evidence based wound healing activity of Lawsonia inermis Linn. Phytother Res (2007) 21(9): 827-31.
[10] Muhammad H, Muhammad S. The use of Lawsonia inermis Linn.(henna) in the management of burn wound infections. Afr J Biotechnol (2005) 4(9).
[11] Parente LM, Lino Júnior RD, Tresvenzol LM, Vinaud MC, de Paula JR, Paulo NM. Wound healing and anti-inflammatory effect in animal models of Calendula officinalis L. growing in Brazil. Evid Based Complement Alternat Med (2012) 2012.
[12] Muley B, Khadabadi S, Banarase N. Phytochemical constituents and pharmacological activities of Calendula officinalis Linn (Asteraceae): a review. Trop J Pharm Res (2009) 8(5).
[13] Parente LM, Andrade MA, Brito LA, Moura VM, Miguel MP, Lino-Júnior RD, Tresvenzol LF, Paula JR, Paulo NM. Angiogenic activity of Calendula officinalis flowers L. in rats. Acta Cir Bras (2011) 26(1):19-24.
[14] Preethi KC, Kuttan G, Kuttan R. Anti-inflammatory activity of flower extract of Calendula officinalis Linn. and its possible mechanism of action. Indian J Exp Biol (2009) 47(2): 113.
[15] Preethi KC, Kuttan R. Wound healing activity of flower extract of Calendula offlcinalis. J Basic Clin Physiol Pharmacol (2009) 20(1): 73-80.
[16] Leach MJ. Calendula officinalis and Wound Healing: A Systematic Review. Wounds: a compendium of clinical research and practice (2008) 20(8): 236-43.
[17] Al-Snafi AE. The chemical constituents and pharmacological effects of Calendula officinalis-A review. Indian Journal of Pharmaceutical Science & Research (2015) 5(3): 172-85.
[18] Re T, Mooney D, Antignac E, Dufour E, Bark I, Srinivasan V, et al. Application of the threshold of toxicological concern approach for the safety evaluation of calendula flower (Calendula officinalis) petals and extracts used in cosmetic and personal care products. Food Chem Toxicol (2009) 47(6): 1246-54.
[19] Pommier P, Gomez F, Sunyach M, D'hombres A, Carrie C, Montbarbon X. Phase III randomized trial of Calendula officinalis compared with trolamine for the prevention of acute dermatitis during irradiation for breast cancer. J Clin Oncol (2004) 22(8): 1447-53.
[20] Lievre M, Marichy J, Baux S, Foyatier J, Perrot J, Boissel J. Controlled study of three ointments for the local management of 2nd and 3rd degree burns. Clin Trial Meta-Anal (1992) 28(1): 9-12.
[21] Tanideh N, Haddadi MH, Rokni-Hosseini MH, Hossienzadeh M, Mehrabani D, Sayehmiri K, Koohi-Hossienabadi O. The healing effect of scrophularia striata on experimental burn wounds infected to pseudomonas aeruginosa in rat. WJPS (2015) 4(1): 16-22.
[22] Mehrabani D, Farjam M, Geramizadeh B, Tanideh N, Amini M, Panjehshahin MR. The healing effect of curcumin on burn wounds in rat. WJPS (2015) 4(1): 29-35.
[23] Hosseini S, Niknahad H, Fakhar N, Rezaianzadeh A, Mehrabani D. The healing effect of mixture of honey, putty, vitriol and olive oil in Pseudomonas aeroginosa infected burns in experimental rat model. Asian J Anim Vet Adv (2011) 6(6): 572-9.
[24] Game et al. IWGDF guidance on use of interventions to enhance the healing of chronic ulcers of the foot in diabetes. Diabetes Metab Rev (2016) 32(1): 75-83.
[25] Leaper D. Appropriate use of silver dressings in wounds: international consensus document. Int Wound J (2012) 9(5): 461-4.
[26] Atiyeh BS, Costagliola M, Hayek SN, Dibo SA. Effect of silver on burn wound infection control and healing: review of the literature. Burns (2007) 33(2): 139-48.
[27] Khanavi M, Vatandoost H, Dehaghi NK, Dehkordi AS, Sedaghat MM, Hadjiakhoondi A, Hadjiakhoondi F. Larvicidal activities of some Iranian native plants against the main malaria vector, Anopheles stephensi. Acta Med Iranica (2013) 51(3): 141.
[28] K. Chandran P, Kuttan R. Effect of Calendula officinalis flower extract on acute phase proteins, antioxidant defense mechanism and granuloma formation during thermal burns. J Clin Biochem Nutr (2008) 43(2): 58-64.
[29] Fronza M, Heinzmann B, Hamburger M, Laufer S, Merfort I. Determination of the wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts. J Ethnopharmacol (2009) 126(3): 463-7.
[2] Tanideh N, Rokhsari P, Mehrabani D, Mohammadi Samani S, Sabet Sarvestani F, Ashraf MJ, Koohi Hosseinabadi O, Shamsian S, Ahmadi N. The healing effect of licorice on Pseudomonas aeruginosa infected burn wounds in experimental rat model. WJPS (2014) 3(2): 99-106.
[3] Sai KP, Babu M. Traditional medicine and practices in burn care: need for newer scientific perspectives. Burns (1998) 24(5): 387-8.
[4] Ashkani-Esfahani S, Imanieh MH, Khoshneviszadeh M, Meshksar A, Noorafshan A, Geramizadeh B, Ebrahimi S, Handjani F, Tanideh N. The healing effect of arnebia euchroma in second degree burn wounds in rat as an animal model. IRAN RED CREACENT MED J (2012) 2012(2): 70-4.
[5] Albertyn R, Berg A, Numanoglu A, Rode H. Traditional burn care in sub-Saharan Africa: a long history with wide acceptance. Burns (2015) 41(2): 203-11.
[6] Stipcevic T, Piljac A, Piljac G. Enhanced healing of full-thickness burn wounds using di-rhamnolipid. Burns (2006) 32(1): 24-34.
[7] Mohsenikia M, Nuraei H, Karimi F, Jamalnia N, Esfahani SA, Rafiee S, Azizian Z, Moradi A. Comparing Effects of Arnebia euchroma and Alpha Ointment on Wound Healing Process. Thrita (2015) 4(1).
[8] Hosseini SV, Tanideh N, Kohanteb J, Ghodrati Z, Mehrabani D, Yarmohammadi H. Comparison between Alpha and silver sulfadiazine ointments in treatment of Pseudomonas infections in 3rd degree burns. Int J Surg (2007) 5(1): 23-6.
[9] Nayak BS, Isitor G, Davis E, Pillai G. The evidence based wound healing activity of Lawsonia inermis Linn. Phytother Res (2007) 21(9): 827-31.
[10] Muhammad H, Muhammad S. The use of Lawsonia inermis Linn.(henna) in the management of burn wound infections. Afr J Biotechnol (2005) 4(9).
[11] Parente LM, Lino Júnior RD, Tresvenzol LM, Vinaud MC, de Paula JR, Paulo NM. Wound healing and anti-inflammatory effect in animal models of Calendula officinalis L. growing in Brazil. Evid Based Complement Alternat Med (2012) 2012.
[12] Muley B, Khadabadi S, Banarase N. Phytochemical constituents and pharmacological activities of Calendula officinalis Linn (Asteraceae): a review. Trop J Pharm Res (2009) 8(5).
[13] Parente LM, Andrade MA, Brito LA, Moura VM, Miguel MP, Lino-Júnior RD, Tresvenzol LF, Paula JR, Paulo NM. Angiogenic activity of Calendula officinalis flowers L. in rats. Acta Cir Bras (2011) 26(1):19-24.
[14] Preethi KC, Kuttan G, Kuttan R. Anti-inflammatory activity of flower extract of Calendula officinalis Linn. and its possible mechanism of action. Indian J Exp Biol (2009) 47(2): 113.
[15] Preethi KC, Kuttan R. Wound healing activity of flower extract of Calendula offlcinalis. J Basic Clin Physiol Pharmacol (2009) 20(1): 73-80.
[16] Leach MJ. Calendula officinalis and Wound Healing: A Systematic Review. Wounds: a compendium of clinical research and practice (2008) 20(8): 236-43.
[17] Al-Snafi AE. The chemical constituents and pharmacological effects of Calendula officinalis-A review. Indian Journal of Pharmaceutical Science & Research (2015) 5(3): 172-85.
[18] Re T, Mooney D, Antignac E, Dufour E, Bark I, Srinivasan V, et al. Application of the threshold of toxicological concern approach for the safety evaluation of calendula flower (Calendula officinalis) petals and extracts used in cosmetic and personal care products. Food Chem Toxicol (2009) 47(6): 1246-54.
[19] Pommier P, Gomez F, Sunyach M, D'hombres A, Carrie C, Montbarbon X. Phase III randomized trial of Calendula officinalis compared with trolamine for the prevention of acute dermatitis during irradiation for breast cancer. J Clin Oncol (2004) 22(8): 1447-53.
[20] Lievre M, Marichy J, Baux S, Foyatier J, Perrot J, Boissel J. Controlled study of three ointments for the local management of 2nd and 3rd degree burns. Clin Trial Meta-Anal (1992) 28(1): 9-12.
[21] Tanideh N, Haddadi MH, Rokni-Hosseini MH, Hossienzadeh M, Mehrabani D, Sayehmiri K, Koohi-Hossienabadi O. The healing effect of scrophularia striata on experimental burn wounds infected to pseudomonas aeruginosa in rat. WJPS (2015) 4(1): 16-22.
[22] Mehrabani D, Farjam M, Geramizadeh B, Tanideh N, Amini M, Panjehshahin MR. The healing effect of curcumin on burn wounds in rat. WJPS (2015) 4(1): 29-35.
[23] Hosseini S, Niknahad H, Fakhar N, Rezaianzadeh A, Mehrabani D. The healing effect of mixture of honey, putty, vitriol and olive oil in Pseudomonas aeroginosa infected burns in experimental rat model. Asian J Anim Vet Adv (2011) 6(6): 572-9.
[24] Game et al. IWGDF guidance on use of interventions to enhance the healing of chronic ulcers of the foot in diabetes. Diabetes Metab Rev (2016) 32(1): 75-83.
[25] Leaper D. Appropriate use of silver dressings in wounds: international consensus document. Int Wound J (2012) 9(5): 461-4.
[26] Atiyeh BS, Costagliola M, Hayek SN, Dibo SA. Effect of silver on burn wound infection control and healing: review of the literature. Burns (2007) 33(2): 139-48.
[27] Khanavi M, Vatandoost H, Dehaghi NK, Dehkordi AS, Sedaghat MM, Hadjiakhoondi A, Hadjiakhoondi F. Larvicidal activities of some Iranian native plants against the main malaria vector, Anopheles stephensi. Acta Med Iranica (2013) 51(3): 141.
[28] K. Chandran P, Kuttan R. Effect of Calendula officinalis flower extract on acute phase proteins, antioxidant defense mechanism and granuloma formation during thermal burns. J Clin Biochem Nutr (2008) 43(2): 58-64.
[29] Fronza M, Heinzmann B, Hamburger M, Laufer S, Merfort I. Determination of the wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts. J Ethnopharmacol (2009) 126(3): 463-7.
- Abstract Viewed: 134 times
- IJPS_Volume 13_Issue 2_Pages 51-60 Downloaded: 50 times