Anti-Melanogenic Activity of Different Extracts from Aerial Parts of Nepeta glomerulosa on Murine Melanoma B16F10 Cells Anti-Melanogenic Activity of Nepeta glomeruloas on Murine Melanoma B16F10 Cells
Iranian Journal of Pharmaceutical Sciences,
Vol. 13 No. 2 (2017),
1 Farvardin 2017
,
Page 61-74
https://doi.org/10.22037/ijps.v13.40698
Abstract
Nepeta glomerulosa Boiss. is a medicinal plant used in traditional medicine. The aim of this study was to evaluate the anti-melanogenesis inhibitory activity of Methanol (MeOH), n-hexane, acetate (EtAc), dichloromethane (CH2Cl2), n-butanol (BuOH), and H2O extracts isolated from N. glomerulosa in B16 melanoma cell line. The B16F10 cell line viability after treatment with increasing concentrations of different extracts of the plant (50-200 μg/ml) was measured by using MTT. The inhibitory effect on synthesis of melanin, mushroom tyrosinase activity, cellular tyrosinase, and effect on oxidative stress was determined by the colorimetric and fluorometric method. The data showed that at concentrations <200 μg/ml, all extracts did not show significant toxicity on melanoma cells. The amount of melanin synthesis by MeOH and CH2Cl2 extracts and mushroom tyrosinase activity by the MeOH extract declined in B16F10 cells. In addition to the capacity of MeOH, n-hexane and CH2Cl2 extracts in decreasing the amount of reactive oxygen species (ROS) in melanoma cells all extracts revealed the remarkable antioxidant activity. Our results showed the melanogenesis inhibitory and antioxidant effects of N. glomerulosa on B16F10 cells may recommend a novel agent in diminishing skin pigmentation and skin aging in cosmetic industry.
Keywords:
- Melanogenesis
- Melanoma
- Nepeta glomerulosa
- Tyrosinase
How to Cite
Seyed Ahmad Emami, Mohammad Sadeghi, Rezvan Yazdian-Robati, Atefeh Arab, & Zahra Tayarani-Najaran. (2017). Anti-Melanogenic Activity of Different Extracts from Aerial Parts of Nepeta glomerulosa on Murine Melanoma B16F10 Cells: Anti-Melanogenic Activity of Nepeta glomeruloas on Murine Melanoma B16F10 Cells. Iranian Journal of Pharmaceutical Sciences, 13(2), 61–74. https://doi.org/10.22037/ijps.v13.40698
References
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[35] Bennett DC, Lamoreux ML. The color loci of mice–a genetic century. Pigment Cell Res. (2003)16 (4):333-344.
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[37] Sasaki M, Kizawa K, Igarashi S, Horikoshi T, Uchiwa H, Miyachi Y. Suppression of melanogenesis by induction of endogenous intracellular metallothionein in human melanocytes. Exp Dermatol (2004)13 (8):465-471.
[38] Schallreuter KU, Wazir U, Kothari S, Gibbons NC, Moore J, Wood JM. Human phenylalanine hydroxylase is activated by H 2 O 2: a novel mechanism for increasing the l-tyrosine supply for melanogenesis in melanocytes. Biochem. Biophys. Res. Commun. (2004) 322 (1):88-92.
[39] Gerlier D, Thomasset N. Use of MTT colorimetric assay to measure cell activation. J Immunol Methods. (1986) 94 (1-2):57-63.
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[42] Masaki H. Role of antioxidants in the skin: anti-aging effects. J Dermatol Sci. (2010) 58 (2):85-90.
[2] Kadekaro AL, Kavanagh RJ, Wakamatsu K, Ito S, Pipitone MA, Abdel‐Malek ZA. Cutaneous photobiology. The melanocyte vs. the sun: who will win the final round? Pigment Cell Res. (2003)16 (5):434-447.
[3] Scott MC, Suzuki I, Abdel‐Malek ZA. Regulation of the human melanocortin 1 receptor expression in epidermal melanocytes by paracrine and endocrine factors and by ultraviolet radiation. Pigment Cell Res. (2002)15 (6):433-439.
[4] Bilodeau ML, Greulich JD, Hullinger RL, Bertolotto C, Ballotti R, Andrisani OM. BMP‐2 stimulates tyrosinase gene expression and melanogenesis in differentiated melanocytes. Pigment Cell Research (2001)14 (5):328-336.
[5] Yamakoshi J, Otsuka F, Sano A, Tokutake S, Saito M, Kikuchi M, et al. Lightening effect on ultraviolet‐induced pigmentation of guinea pig skin by oral administration of a proanthocyanidin‐rich extract from grape seeds. Pigment Cell Res. (2003)16 (6):629-638.
[6] Seo S-Y, Sharma VK, Sharma N. Mushroom tyrosinase: recent prospects. J AGR FOOD CHEM. (2003)51 (10):2837-2853.
[7] Hearing VJ, Jiménez M. Mammalian tyrosinase—the critical regulatory control point in melanocyte pigmentation. International Journal of Biochemistry. (1987)19 (12):1141-1147.
[8] Tsukamoto K, Jackson IJ, Urabe K, Montague PM, Hearing V. A second tyrosinase-related protein, TRP-2, is a melanogenic enzyme termed DOPAchrome tautomerase. The EMBO Journal. (1992)11 (2):519.
[9] Battaini G, Monzani E, Casella L, Santagostini L, Pagliarin R. Inhibition of the catecholase activity of biomimetic dinuclear copper complexes by kojic acid. J Biol Inorg Chem. (2000)5 (2):262-268.
[10] Lee CH, Seib PA, Liang YT, Hoseney RC, Deyoe CW. Chemical synthesis of several phosphoric esters of L-ascorbic acid. Carbohydr. Res. (1978) 67 (1):127-138.
[11] Hosoi J, Abe E, Suda T, Kuroki T. Regulation of melanin synthesis of B16 mouse melanoma cells by 1α, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res. (1985) 45 (4):1474-1478.
[12] Jimbow K, Minamitsuji Y. Topical therapies for melasma and disorders of hyperpigmentation. Dermatol Ther. (2001)14 (1):35-45.
[13] Nakajima M, Shinoda I, Fukuwatari Y, Hayasawa H. Arbutin increases the pigmentation of cultured human melanocytes through mechanisms other than the induction of tyrosinase activity. Pigment cell Research (1998)11 (1):12-7.
[14] Hwang J-H, Lee BM. Inhibitory effects of plant extracts on tyrosinase, L-DOPA oxidation, and melanin synthesis. J.Toxicol and Environ. Health, Part A. (2007)70 (5):393-407.
[15] No JK, Kim YJ, Shim KH, Jun YS, Rhee SH, Yokozawa T, et al. Inhibition of tyrosinase by green tea components. Life sciences (1999) 65 (21):PL241-PL246.
[16] Palumbo A, d'Ischia M, Misuraca G, Prota G. Mechanism of inhibition of melanogenesis by hydroquinone. BBA-General Subjects (1991)1073 (1):85-90.
[17] Jones K, Hughes J, Hong M, Jia Q, Orndorff S. Modulation of melanogenesis by aloesin: a competitive inhibitor of tyrosinase. Pigment Cell Res ( 2002)15 (5):335-340.
[18] Kim YJ, No JK, Lee JH, Chung HY. 4, 4'-Dihydroxybiphenyl as a new potent tyrosinase inhibitor. Biol Pharm Bull (2005) 28 (2):323-327.
[19] Takizawa T, Mitsumori K, Tamura T, Nasu M, Ueda M, Imai T, et al. Hepatocellular tumor induction in heterozygous p53-deficient CBA mice by a 26-week dietary administration of kojic acid. Toxicol Sci. (2003)73 (2):287-293.
[20] Frenk E. Treatment of melasma with depigmenting agents. Melasma: new approaches to treatment. (1995) 9-15.
[21] Hermanns J, Petit L, Martalo O, Pierard-Franchimont C, Cauwenbergh G, Pierard G. Unraveling the patterns of subclinical pheomelanin-enriched facial hyperpigmentation: effect of depigmenting agents. Dermatol. (2000) 201 (2):118-122.
[22] Mabberley DJ. Mabberley’s plant-book. (2008).
[23] Jamzad Z, Chase MW, Ingrouille M, Simmonds MS, Jalili A. Phylogenetic relationships in Nepeta L.(Lamiaceae) and related genera based on ITS sequence data. Taxon. (2003) 21-32.
[24] Jamzad Z. Lamiaceae. In: Assadi M. ed. Flora of Iran: Tehran: RIFR. (2012).
[25] Emami S, Aghazari F, Johartchi M. Les Phanerogames Éndemiques de la Flore d'Iran: L'Université de Téhéran des Sciences Médicales, Institut des Études d'Histoire de la Médecine, de Médecine Islamique et de Médecine Complémentaire (2011).
[26] Aydin S, Demir T, Oeztuerk Y, Başer KHC. Analgesic activity of Nepeta italica L. Phytother Res. (1999)13 (1):20-3.
[27] Ganzera M, Crockett S, Tellez M, Khan I. Determination of nepetalactone in Nepeta cataria by reversed phase high performance liquid chromatography. Die Pharmazie. (2001)56 (11):896.
[28] Sonboli A, Salehi P, Yousefzadi M. Antimicrobial activity and chemical composition of the essential oil of Nepeta crispa Willd. from Iran. Zeitschrift für Naturforschung C. (2004)59 (9-10):653-656.
[29] Hosseini A, Forouzanfar F, Rakhshandeh H. Hypnotic effect of Nepeta glomerulosa on pentobarbital-induced sleep in mice. JUNDISHAPUR J NAT PHARM PROD. (2016)11 (1). jjnpp-25063.
[30] Tada H, Shiho O, Kuroshima K-i, Koyama M, Tsukamoto K. An improved colorimetric assay for interleukin 2. J Immunol Methods (1986) 93 (2):157-165.
[31] Hyun SK, Lee W-H, Jeong DM, Kim Y, Choi JS. Inhibitory effects of kurarinol, kuraridinol, and trifolirhizin from Sophora flavescens on tyrosinase and melanin synthesis. Biol Pharm Bull (2008) 31 (1):154-158.
[32] Kamagaju L, Morandini R, Bizuru E, Nyetera P, Nduwayezu JB, Stévigny C, et al. Tyrosinase modulation by five Rwandese herbal medicines traditionally used for skin treatment. J Ethnopharmacol (2013)146 (3):824-834.
[33] Yokozawa T, Kim YJ. Piceatannol inhibits melanogenesis by its antioxidative actions. Biol Pharm Bull (2007)30 (11):2007-2011.
[34] Farombi EO. African indigenous plants with chemotherapeutic potentials and biotechnological approach to the production of bioactive prophylactic agents. AJB. (2004) 2 (12):662-671.
[35] Bennett DC, Lamoreux ML. The color loci of mice–a genetic century. Pigment Cell Res. (2003)16 (4):333-344.
[36] Curto EV, Kwong C, Hermersdörfer H, Glatt H, Santis C, Virador V, et al. Inhibitors of mammalian melanocyte tyrosinase: in vitro comparisons of alkyl esters of gentisic acid with other putative inhibitors. Biochem Pharmacol. (1999) 57 (6):663-672.
[37] Sasaki M, Kizawa K, Igarashi S, Horikoshi T, Uchiwa H, Miyachi Y. Suppression of melanogenesis by induction of endogenous intracellular metallothionein in human melanocytes. Exp Dermatol (2004)13 (8):465-471.
[38] Schallreuter KU, Wazir U, Kothari S, Gibbons NC, Moore J, Wood JM. Human phenylalanine hydroxylase is activated by H 2 O 2: a novel mechanism for increasing the l-tyrosine supply for melanogenesis in melanocytes. Biochem. Biophys. Res. Commun. (2004) 322 (1):88-92.
[39] Gerlier D, Thomasset N. Use of MTT colorimetric assay to measure cell activation. J Immunol Methods. (1986) 94 (1-2):57-63.
[40] Galato D, Ckless K, Susin MF, Giacomelli C, Ribeiro-do-Valle RM, Spinelli A. Antioxidant capacity of phenolic and related compounds: correlation among electrochemical, visible spectroscopy methods and structure–antioxidant activity. Redox Report. (2001) 6 (4):243-250.
[41] Ryu A, Arakane K, Koide C, Arai H, Nagano T. Squalene as a target molecule in skin hyperpigmentation caused by singlet oxygen. Biol Pharm Bull. (2009) 32 (9):1504-1509.
[42] Masaki H. Role of antioxidants in the skin: anti-aging effects. J Dermatol Sci. (2010) 58 (2):85-90.
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