The Modulating Effect of Glucocorticoids and Opioid System on Anxiety Related Behavior in Young and Adult Rats Economic Evaluation of Infliximab in UC Patients
Iranian Journal of Pharmaceutical Sciences,
Vol. 12 No. 4 (2016),
1 Mehr 2016
,
Page 43-54
https://doi.org/10.22037/ijps.v12.40712
Abstract
One of the main components of the stress system is hypothalamus- pituitary-adrenal (HPA) axis. Acute activation of μ-opioid receptors increases the activity of the HPA axis, leading to release of ACTH and corticosterone. Glucocorticoids can change behaviors, depend on age but there were no evidences about the interaction between age, opioid system and glucocorticoids. In this experiment, effects of dexamethasone (1mg/kg) and RU486 (20mg/kg) as an agonist and antagonist of glucocorticoid receptors, morphine (5mg/kg) and naloxone (20mg/kg) as an agonist and antagonist of the opioid system on anxiety in young and adult male Wistar rats were examined. The percentage of time in the open arms of plus maze was evaluated for anxiety behavior also percentage of the number of entries in closed arms was evaluated for locomotor activity. The results showed that morphine (5mg/kg) and dexamethasone (1mg/kg) had an anxiolytic effect on both young and adult rats while just in young rats reduced locomotor activity. RU486 could prevent the anxiolytic effect of morphine, and the anxiolytic effect of dexamethasone had been inhibited by naloxone in young but it wasn't seen in adult rats. These results show an interactive effect between glucocorticoids and the opioid system on mediating anxiety that can be influenced by age.
- anxiety
- glucocorticoid
- mice
- opioid
- RU486
How to Cite
References
[2] Etim NN, Evans EI, Offiong EEA, Williams ME. Stress and the neuroendocrine system: implications for animal well-being. Am J Biol Life Sci (2013) 1(1): 20-26.
[3] Tsigos C, Chrousos G. Hypothalamic-pituitary-adrenal axis, neuroendocrine factors and stress. J Psychosom Res (2002) 53(4): 865-871.
[4] Boyle MP, Kolber BJ, Vogt S, Wozniak D, Muglia L. Forebrain Glucocorticoid Receptors Modulate Anxiety-Associated Loco-motors Activation and Adrenal Responsiveness. J Neurosci (2006) 26(7): 1971-1978.
[5] Velisek L. Prenatal exposure to beta-methasone decreases anxiety in developing rats: hippocampal neuropeptide y as a target molecule. Neuropsychopharmssacology (2006) 31(10): 2140-2149.
[6] Vafaei AA, Rashidi-pour A, Taherianpak AA. Peripheral injection of dexamethasone modulates anxiety related behaviors in mice: an interaction with opioidergic neurons. Pak J Pharmss Sci (2008) 21 (3): 285-289.
[7] Anand R, Gulati K, Ray A. Pharmssacological evidence for the role of nitric oxide in the modulation of stress-induced anxiety by morphine in rats. Eur J Pharmssacol (2012) 676 (1-3): 71-74.
[8] Rezayof A, Assadpour S, Alijanpour S. Morphine-induced anxiolytic-like effect in morphine-sensitized mice: involvement of ventral hippocampal nicotinic acetylcholine receptors. Pharmssacol Biochem Behav (2013) 103(3): 460-466.
[9] Torabi M, Kesmati M, Harooni HE, Najafzadeh varzi H. Effect of intra CA1 and intraperitoneal administration of opioid receptor modulating agents on the anxiolytic properties of nano and conventional ZnO in male rats. Cell J (2014) 16(2): 163-170.
[10] Bodnar RJ. Endogenous opiates and behavior: 2013. Peptides (2014) 62: 67-136 [11] Amiri S, Divanbeigi A. P80: Investigation of Interactions between Glucocorticoid and Opioid Receptors on Anxiety Modulation in Mice. Shefaye Khatam (2014) 2 (S1): 104-104.
[12] Zhang GF, et al. Dysfunction of the hypothalamic-pituitary-adrenal axis in opioid dependent subjects: effects of acute and protracted abstinence. Am J Drug Alcohol Abuse (2008) 34(6):760-768.
[13] Lim G, Wang S, Zeng Q, Sung B, Mao J. Spinal glucocorticoid receptors contribute to development of morphine tolerance in rats. Anesthesiology (2005) 102(4): 832-837.
[14] Millanes MV, Laorden ML, Chapleur-Chateau M, Bulert A. Differential regulation of corticotrophin-releasing factor and vasopressin in discrete brain regions after morphine administration: correlation with hypothalamic noradrenergic activity and pituitary adrenal response. Naunyn Schmiedebergs Arch Pharmssacol (1997) 356(5): 603-610.
[15] Nock B, Cicero T, Wich M. Chronic morphine increases the pituitary-adrenocortical response of juvenile rats to mild stress. Pharmssacol Biochem Behav (2005) 80( 1): 77-85.
[16] Bennett K, et al. Cognitive behavioral therapy age effects in child and adolescent anxiety: an individual patient data metaanalysis. Depress Anxiety (2013) 30 (9): 829-841.
[17] Elberta GJ. Age-related social, emotional, and behavioral differences in children and adolescents manifesting the symptom presentation of nonverbal learning disabilities." PhD (Doctor of Philosophy) thesis, University of Iowa, 2012. http://ir.uiowa.edu/etd/3300.
[18] Koukouli S, Pattakou-Parasyri V, Kalaitzaki AE. Self-Reported Aging Anxiety in Greek Students, Health Care Professionals, and Community Residents: A Comparative Study. Gerontologist (2013) 54(2): 201-210.
[19] Bessa JM, Olivera M, Cerqueira JJ, Almedia OFX, Sousa N. Age-related qualitative shift in emotional behaviour: Paradoxical findings after re-exposure of rats in the elevated-plus maze. J Behave Brain Res (2005)162(1): 135-142.
[20] Wehry AM, Beesdo-Baum K, Hennelly MM, Connolly SD, Strawn JR. Assessment and treatment of anxiety disorders in children and adolescents. Curr Psychiatry Rep (2015) 17(7): 591.
[21] Kamphuis PJ, Croiset G, Bakker JM, Van Bel F, Van Ree JM, Wiegant VM. Neonatal dexamethasone treatment affects social behaviour of rats in later life. Neuropharmssacology (2004) 47(3): 461-474.
[22] Dieleman GC, Huizink AC, Tulen JH, Utens EM, Creemers HE, van der Ende J, Verhulst FC. Alterations in HPA-axis and autonomic nervous system functioning in childhood anxiety disorders point to a chronic stress hypothesis. Psychoneuroendocrinology (2015) 51: 135-50.
[23] Lim G, Wang S, Zeng Q, Sung B, Yang L, Mao J. Expression of spinal NMDA receptor and PKCgamma after chronic morphine is regulated by spinal glucocorticoid receptor. J Neurosci (2005) 25(48): 11145-11154.
[24] Uriguen L, et al. Effects of 14-methoxymetopon, apotent opioid agonist, on the responses to the tail electric stimulation test and plus-maze activity in male rats: Neuroendocrine correlates. Brain Res Bull (2002) 57(5): 661-666.
[25] Laue L, Chrousos GP, Loriaux DL, Barnes K, Munson P, Nieman L, Schaison G. The antiglucocorticoid and antiprogestin steroid RU 486 suppresses the adrenocorticotropin response to ovine corticotropin releasing hormone in man. J Clin. Endocrinol Metab (1988) 66(2): 290-293.
[26] Rashidy-Pour A, Sadeghi H, Taherian AA, Vafaei AA, Fathollahi Y. The effects of acute restraint stress and dexamethasone on retrieval of long-term memory in rats: an interaction with opiate system. Behav Brain Res (2004) 154(1): 193-198.
[27] Ferguson S, Gray E. Aging effects on elevated plus maze behavior in spontaneously hypertensive, Wistar-Kyoto and Sprague-Dawley male and female rats. Physiol Behave (2005) 85(5): 621-628.
[28] Arrant AE, Schramm-Sapyta NL, Kuhn CM. Use of the Light/Dark Test for Anxiety in Adult and Adolescent Male Rats. Behav Brain Res (2013) 256: 119-127.
[29] Mikics E, Barsy B, Barsvari B, Haller J. Behavioral specificity of non-genomic glucocorticoid effects in rats: Effects on risk assessment in the elevated plus-maze and open-field. Horm Behav (2005) 48(2): 152-162.
[30] Fuertes G, Milanes M, Rodriguez-Gago M, Martin MT, Laorden ML. Changes in hypothalamic paravenricular nucleus catecholaminergic activity after acute and chronic morphine administration.
Eur J Pharmssacol (2000) 388(1): 49-56.
[31] Maggi R, Pimpinelli F, Casulari L, Martini F. Antiprogestins inhibit the binding of opioids to μ-opioid receptors in nervous membrain preparations. Eur J Pharmssacol (1996) 301(1-3): 169-177.
[32] Pivina SG, Akulova VK, Ordian NE. The impact of early developmental impairment of the receptor-dependent glucocorticoid action on the pituitary adrenal axis activity and behavior of male rats. Ross Fiziol Zh Im I M Sechenova (2010) 96(1): 69-76.
[33] Campo M, Sotres J, Ferra K, Aguirre A. High dose naloxone (1mg/kg): Psychological and endocrine effects in normal male subjects pretreated with one milligram of dexamethasone. Psychoneuroendocrinol (1998) 23(4): 413-424.
[34] Evans SJ, Murray TF, Moore FJ. Partial purification and biochemical characterization of a membrane glucocorticoid receptor from an amphibian brain. J Steroid Biochem Mol Biol (2000) 72(5): 209-221
- Abstract Viewed: 73 times
- IJPS_Volume 12_Issue 4_Pages 43-54 Downloaded: 24 times