A Comparative Study on the Effects of Ziziphus Spina-christi Alcoholic Extracts on Growth and Structural Integrity of Bacterial Pathogens Ziziphus spina-christi as a native antibacterial plant
Iranian Journal of Pharmaceutical Sciences,
دوره 10 شماره 2 (2014),
1 فروردین 2014
,
صفحه 1-10
https://doi.org/10.22037/ijps.v10.40820
چکیده
In different folk medicine Ziziphus spina-christi is used for different purposes such as pneumonia, dysentery, scorpion stings, cough, constipation, intestinal worms and fever. The aim of this study was evaluation and comparing the antibacterial activity of methanolic and ethanolic extracts of Ziziphus spina –christi as well as subsequent structural changes in affected bacteria. For this purpose, ethanolic and methanolic extracts were prepared by 80% alcoholic solution. Antibacterial activity of these extracts was assessed using standard disc diffusion method against pathogenic bacteria. Sterile filter paper discs (6mm) were saturated by four different concentrations of each extract. The prepared discs were placed on lawn cultures of test bacteria and incubated at 37 0C for 24 h. After incubation the inhibition zone diameter around each disc was measured in millimeter. The induced changes in shape of affected bacteria were discovered using scanning electron microscopy (SEM). As a result of this study maximum
inhibition zone diameter in case of methanolic extract were 18 and 14 against Staphylococcus aureus, Bacillus cereu, and in case of ethanolic extract was 15 mm for S. aureus and Proteus mirabilis. The methanolic extract of this plant was more effective against S. aureus and B. cereus than the ethanolic extract even at high concentration. While the ethanolic extract was more active on Proteus mirabilis. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) indexes of both extracts were equal (MIC= MBC=8 mg/ml) for S. aureus. The SEM analysis revealed cell deformation and irregular shape in both S. aureus and B. cereus. These results suggest significant antibacterial activity of this plant especially against S. aureus, which its resistant strains are currently a great hazard in infection treatment. So, this plant should be considered as a potential source for finding new antibacterial agents.
- antibacterial activity
- ethanolic extract
- medicinal plant
- methanolic extract
- MIC
- MBC
- Ziziphus spinachristi
ارجاع به مقاله
مراجع
[2] Waggas A M. Acute effect of Sidr leaves extract on some neurotransmitter Contents in different brain areas of male albino rats. Saudi J Biol Sci (2007) 14 (1) 93-101.
[3] Abalaka ME, Daniyan SY, Mann A. Evaluation of the antimicrobial activities of two Ziziphus species (Ziziphus mauritiana L. and Ziziphus spinachristi L.) on some microbial pathogens. Afr J Pharm Pharmacol (
2010) 4 (4):135-139.
[4] Waggas A M, Al-Hasani R H. Effect of Sidr (Zizyphus spina-christi ) Fruit Extract on the central nervous system in male albino rats. Am- Euras J Sci Res (2009) 4 (4): 263-267.
[5] Motamedi H, Safary A, Maleki S, Seyyednejad SM.Ziziphus spina-christi a native plant from Khuzestan, Iran, as a potential source for discovery new antimicrobial agents. Asian J Plant Sci (2009) 8 (2):187-190.
[6] Adzu B, Haruna AK. Studies on the use of Zizyphus spina-christi against pain in rats and mice. Afri J Biotechnol (2007) 6 (11):1317-1324.
[7] Abu-Taleb A M, El- Deeb K, Al-Otibi F O.Assessment of antifungal activity of Rumex vesicarius L. and Ziziphus spina-christi L. Willd extracts against two phytopathogenic fungi. Afr J Microbiol Res (2011)
5(9) 1001-1011.
[8] Dahiru D, Sini J M, John-Africa L. Antidiarrhoeal activity of Ziziphus mauritiana root extract in rodents. Afr J Biotechnol ( 2006) 5 (10): 941-945.
[9] Bakkali F, Averbeck S, Averbeck D, Idaomar M.Biological effect of essential oils- a review. Food Chem Toxicol (2008) 46(2):446-475.
[10] Ghasemi Pirbalouti A, Bahmani M, Avijgan M.Anti-Candida Activity of Some of the Iranian Medicinal Plants. E J Biol ( 2009) 5 (4):85-88.
[11] Shojaai M, Maleki S, Darabpour E, Motamedi H, Seyyednejad SM. Antibacterial activity of eight Iranian plant extracts against methicillin and cefixime resistant Staphylococous aureus strain. Asian Pac J Trop Med (2010) 3(4): 262-265.
[12] Seyyednejad SM, Koochak H, Darabpour E, Motamedi H. A survey on Hibiscus rosa-sinensis, Alcea rosea L. and Malva neglecta wallr as antibacterial agents. Asian Pac J Trop Med (2010) 3(5): 351-355.
[13] Motamedi H, Darabpour E, Gholipour M, Seyyednejad SM. Antibacterial effect of ethanolic and methanolic extracts of Plantago ovate and Oliveria decumbens endemic in Iran against some pathogenic
bacteria. Int J Pharmacol (2010) 6 (2): 117-122.
[14] Seyyednejad SM, Niknejad M, Darabpoor E, Motamedi H. Antibacterial activity of Hydroalcoholic extract of Callistemon citrinus and Albizia lebbeck . Am J Applied Sci (2010) 7 (1):13-16.
[15] Nisar M, Kaleem WA, Qayum M, Hussain A, Ziaul haq M, Ali I, Choudhary MI. Biological screening of Zizyphus oxyphylla edgew leaves. Pak J Bot (2010) 42 (6):4063-4069.
[16] Mohamed IET, El Sheikh El Nur E B, Abdelrahman M E N. The antibacterial, antiviral activities and phytochemical screening of some Sudanese medicinal plants. EurAsia J BioSci (2010) 4 (2): 8-16.
[17] Mahesh B. and Satish S. Antimicrobial Activity of Some Important Medicinal Plant against Plant and Human Pathogens. World J Agri Sci ( 2008) 4(S): 839-843.
[18] Cogo L L, Bastos Monteiro CL, Dallarmi Miguel M, Gomes Miguel O, Machado Cunico M, Lima Ribeiro L, Camargo ER, Botao Kussen GM, Silva Nogueira K, Dalla Costa LM. Anti- Helicobacter pylori activity of plant extracts traditionally used for the treatment of gastrointestinal disorders. Braz J Microbiol (2010) 41(2): 304-309.
- چکیده مشاهده شده: 124 بار
- IJPS_Volume 10_Issue 2_Pages 1-10 (English) دانلود شده: 41 بار