Comparison of the Cytotoxic Effects of Nanosilver and Ancient Iranian and Pakistani Silver Kushtas on Primary Rat Hepatocytes and Human Cancer Cell Lines Comparison of Cytotoxic Effects of Nanosilver and Ancient Iranian and Pakistani Silver Kushtas
Iranian Journal of Pharmaceutical Sciences,
Vol. 10 Núm. 2 (2014),
1 April 2014
,
Página 35-46
https://doi.org/10.22037/ijps.v10.40825
Resumen
Tumor growth is characterized by uncontrolled cell division. For centuries, silver kushta powder, composed of nano and submicro silver particles, has been used in traditional Iranian and Pakistani medicine for the treatment of melanoma and breast cancer. We have found that these nanocomposite particles are similar to silver nanoparticles (AgNps) in size and shape and that there are so differences in their physicochemical properties such as silver content.In the present study, a comparison of cytotoxic effects of nanosilver(AgNps) and two silver kushtas [Iranian silver kushta (IKAg) and Pakistan silver kushta (PKAg)]have been conductedindifferent concentrations against tumor cell lines (MCF-7, HepG2, A549) and a normal cell line (MDBK) using MTT andTrypan blue exclusion tests.At first, Particle size was analyzed using the Malvern Zetasizer. The Z average diameters of samples (AgNps, PKAg, and IKAg) were64.08, 51.72 and 190.4 nm, respectively.The result of MTTtest showed no toxicity of both silver kushtas (IKAg&PKAg) toward the cancer cell lines and MDBK cells. The IC50 values of AgNpsdetermined for A549, HPG2, MCF-7, and MDBK were 5.94, 1.41, 3.68, and, 1.9 ppm, respectively. According to trypan blue (0.2% w/v) exclusion test, the cytotoxicity of the silver materials toward primary rat hepatocytes followed the order AgNps (100%) >AgNO3 (80.9%) >Pakistani silver kushta (48.35%)>Iranian silver kushta (45%).This result illustrated that the silver components [(IKAg) & (PKAg)] of the traditional kushtas do not penetrate the cancer cell membrane and do not show cytotoxicity. Therefore, kushtas are ineffective as anti-cancer agents. However, AgNps shows good anticancer properties.
- Cancer
- Cell line
- Cytotoxcity
- Kushtas
- MTT assay
- Particle size
- Trypan blue.
Cómo citar
Citas
[2]Avicenna. The Canon of Medicine. Trans. SharafkandiA. 7th Pub. (2008)6:410.
[3]AghiliAlaviKhorasaniShirazi SMH. Makhzanul- Advia [In Persian]. The Institue of Medical history, Islamic and complementary medicine studies: Tehran (2008):439.
[4]MomenTonekaboni M. Tohfat al Momenin.Rahimi, R., (Eds.) MoaseseNashreShahr. Tehran (2008): 279.
[5] Galabuzi C, Agea J, Fungo B, Kamoga R. Traditional medicine as an alternative form of health care system: a preliminary case study of Nangabo sub-county, central Uganda. Afr J Tradit Complement Altern Med (2010): 7.
[6] Kayne S. Introduction to traditional medicine. Traditional medicine: A global perspective (2010):1-24.
[7] Bodeker G: Lessons on integration from the developing world's experience. BMJ: British Medical Journal (2001)322:164.
[8] Keen R, Deacon A, Delves H, Moreton J, Frost P. Indian herbal remedies for diabetes as a cause of lead poisoning. Postgrad Med J (1994)70:113-114.
[9] Vohora SB, Athar M.Mineral drugs used in Ayurveda and Unani medicine.Narosa Publishing House (2008).
[10] Avicenna. The Canon of Medicine. Trans. SharafkandiA. 7th Pub. (2008)5:89, 265.
[11] Avicenna. The Canon of Medicine. Trans. SharafkandiA. 7th Pub. (2008)6:408, 409, 386, 457.
[12] Avicenna. The Canon of Medicine. Trans. SharafkandiA. 7th Pub. (2008)7:175, 185, 249, 279, 287.
[13] Avicenna. The Canon of Medicine. Trans. SharafkandiA. 7th Pub. (2008)2:273, 294.
[14] Hakim Abdolvahid and Hakim Sediqi.A survey of Drug, India (1985):7-18.
[15] Abobakr Mohammad IbnZakaria.A brief of Alhavi.Rev. Tabatabaei SM, Mashhad University of Medial Sciences Pub.(2011)3:236, 273, 277, 287.
[16] Glazier A. A landmark in the history of Ayurveda. Lancet(2000)356:1119.
[17] Kew J, Morris C, Aihie A, Fysh R, Jones S, Brooks D. Arsenic and mercury intoxication due to Indian ethnic remedies. Bmj (1993)306:506-507.
[18] Keen R, Deacon A, Delves H, Moreton J, Frost P. Indian herbal remedies for diabetes as a cause of lead poisoning. Postgrad Med J (1994)70:113-114.
[19] Aslam M, Davis S, Healy M. Heavy metals in some Asian medicines and cosmetics. Public Health (1979)93:274-284.
[20] Saper RB, Phillips RS, Sehgal A, Khouri N,Davis RB, Paquin J, Thuppil V, Kales SN. Lead, mercury, and arsenic in US-and Indian-manufactured Ayurvedic medicines sold via the Internet. Jama (2008)300:915-923.
[21] ChristopherGB,BrownL, Whitehouse MW, Agrawal DS, TupeSG, PaknikarKM,TiekinkERT. Nanogold pharmaceutics the use of colloidal gold to treat experimentally-induced arthritis in rat models; Characterization of the gold in SwarnaBhasma, a microparticulate used in traditional Indian medicine.Gold Bulletin(2007)40:6.
[22] Kreuter J. Nanoparticles-a historical perspective. Int J Pharm(2007)331:1-10.
[23] Chaloupka K, Malam Y, Seifalian AM. Nanosilver as a new generation of nanoproduct in biomedical applications. Trends Biotechnol (2010)28:580-588.
[24] Arora S, Jain J, Rajwade J, Paknikar K. Interactions of silver nanoparticles with primary mouse fibroblasts and liver cells. Toxicol Appl Pharm (2009)236:310-318.
[25] Chen X, Schluesener H. Nanosilver: a nanoproduct in medical application. ToxicolLett(2008)176:1-12.
[26] Nowrouzi et al. Cytotoxicity of subtoxic AgNpin human hepatoma cell line (HepG2) after long-term exposure. Iran Biomed J(2010)14:23-32.
[27] Sriram MI, Kanth SBM, Kalishwaralal K, Gurunathan S. Antitumor activity of silver nanoparticles in Dalton’s lymphoma ascites tumor model. Int J Nanomedicine(2010)5:753.
[28] Guzmán MG, Dille J, Godet S. Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity. Int J Chem Biomol Eng (2009)2:104-111.
[29] Hosseini M-J, Seyedrazi N, Shahraki J, Pourahmad J. Vanadium induces liver toxicity through reductive activation by glutathione and mitochondrial dysfunction. AdvBiosciBiotechnol (2012)3(8):1096-1103.
[30] Auffan M, Rose J, Bottero JY, Lowry GV, Jolivet JP, Wiesner MR. Towards a definition of inorganic nanoparticles from an environmental, health and safety perspective. Nat Nanotechnol (2009)4(10):634–641.
[31] Kim S, Choi JE, Choi J, Chung K-H, Park K, Yi J, Ryu D-Y. Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells. Toxicol In vitro (2009)23:1076-1084.
[32] Navarro et al. Toxicity of silver nanoparticles to Chlamydomonas reinhardtii. Environ Sci Technol (2008)42:8959-8964.
[33] Foldbjerg R, Dang DA, Autrup H. Cytotoxicity and genotoxicity of silver nanoparticles in the human lung cancer cell line, A549. Arch Toxicol (2011)85:743-750.
[34] Limbach LK, Wick P, Manser P, Grass RN, Bruinink A, Stark WJ. Exposure of engineered nanoparticles to human lung epithelial cells: influence of chemical composition and catalytic activity on oxidative stress. Environ SciTechnol (2007)41:4158-4163.
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