Evaluation of Anti-hyperglycemic potential of Bauhinia Tomentosa standardized extracts in streptozotocin- induced diabetic rats Fingerprinting and Anti-diabetic analysis of alcoholic and aqueous stem extracts B. tomentosa
Iranian Journal of Pharmaceutical Sciences,
Vol. 10 No. 1 (2014),
15 January 2014
,
Page 1-14
https://doi.org/10.22037/ijps.v10.40830
Abstract
In India, the occurrence of diabetes mellitus is on increase and needs to be addressed properly. Herbal remedies are considered most suitable for the management of type 2 diabetes due to their traditional acceptability and availability, low costs, and fewer side effects. Traditionally Bauhinia tomentosa L. (Family- Fabaceae) is used in the treatment of diabetes in India. Aqueous extract and alcoholic extract (250 and 500 mg/kg) of stem were taken to evaluate the anti-diabetic activity in normal and streptozotocin (65 mg/kg) induced diabetic rats. After 21 days of oral administration of extracts resulted in the significance reduction in blood glucose level. Serum biochemistry showed that serum cholesterol, triglyceride, HDL, LDL levels were significantly decreased by both the extracts and standard drug glibenclamide (10 mcg/kg). HPLC and HPTLC analysis of alcoholic extract found to be rich in flavanoids and phenolic constituents whereas aqueous extract found to possess rich in flavanoids, phenolic constituents, tannins and glycosides. The antidiabetic activity of Bauhinia tomentosa L. may be attributed to its high phenolic and flavanoids constituents.
- Antidiabetic activity
- HDL
- HPTLC
- LDL
- serum cholesterol
- streptozotocin
- triglyceride
How to Cite
References
[2]Kumar P, Baraiya S, Gaidhani SN, Gupta MD, Manish MW. Anti-diabetic activity of stem bark of Bauhinia variegata in alloxan-induced hyperglycemic rats. J Pharmacol Pharmacother (2012)3(1): 64–66.
[3] Lino CS, Diógenes JP, Pereira BA, Faria RA, Andrade NM, Alves RS etal. Anti-diabetic activity of Bauhinia forficata extracts in alloxan-diabetic rats. Biol Pharm Bull (2004)27(1): 125-127.
[4] Chopra RN, Handa KL. Indigenous Drugs of India. Academic Publishers: Kolkata (1982).
[5] Kameswara RB, Giri R, Kesavulu MM, Apparao C. Herbal medicine: in the management of diabetes mellitus. Manphar Vaidhya Patrika (1997)4(5): 33–35.
[6] Day C. Traditional plant treatment for diabetes mellitus: pharmaceutical foods. BRIT J Nutr (1998)80: 5–6.
[7] Dhar ML, Dhawan M, Melhrotra CL. Screening of Indian plants for biological activity Part I. Indian J Exp Biol (1968) 6: 232.
[8] Kirtikar KR, Basu BD. Indian Medicinal Plants. Lalit Mohan Publication: India (1980).
[9] Sammbamurthy AVSS. Dictionary of medicinal plants. CBS Publishers: New Delhi (2006).
[10] Swarnalatha D, Madhu KB, Satayanarayan T, Mallikarjuna RP. Antimicrobial activity of Bauhinia tomentosa and Bauhinia vahlii root. Pharmacog Mag (2010)6: 204.
[11]Tiwari V, Singh A. Elucidation of Possible Mechanism of anti nociceptive and antioxidant potential of Bauhinia tomentosa extracts in experimental animal models. Nat Prod J (2013)3(4): 309-316.
[12] Anonymous. The Wealth of India: Raw Materials. Publications and Information Directorate, CSIR: New Delhi (1948).
[13] Cunha AM, Menon S, Menon R, Couto AG, Burger C, Biavatti MW. Hypoglycemic activity of dried extracts of Bauhinia forficata Link. Phytomed (2010)17(1): 37-41.
[14]Azevedo CR, Macielsilva FM, Silva LB, Ferreira ATS, Cunha MD, Machado OLT et al. Isolation and intracellular localization of insulin-like proteins from leaves of Bauhinia variegata. Braz J Med Biol Res (2006)39: 1435.
[15] Sharanabasappa GK, Santosh MK, Shaila D, Seetharam YN, Sanjeevarao I. Phytochemical Studies on Bauhinia racemosa Lam., Bauhinia purpurea L and Hardwickia binata Roxb. E-Journal Chem (2007)4: 21. [16] Krebs KG, Heunsen D, Stahl WH. Thin layer chromatography - A laboratory hand book. Springer: Berlin (2005).
[17] Tiwari V, Tiwari A, Madhavan V. Preliminary phytochemical analysis, hptlc studies and antipyretic activity of alcohol and aqueous extract of Helicteres isora L root. Int J Pharm Pharm Sci (2010)2: 74.
[18]Tiwari V, Singh A, Tiwari A. Pharmacognostical, Phytochemical, antimicrobial evaluation of Bauhinia tomentosa stem. J Pharm Res (2011)4(4): 1173-1175.
[19]Wagner H, Baldt S, Zgainski EM. Plant drug analysis. Springer: Berlin (1996).
[20] Bhaskar VH, Balakrishnan N. Analgesic, anti-inflammatory and antipyretic activities of Pergularia daemia and Carissa carandas. DARU J Pharm Sci (2009)17: 168.
[21] Friedwald J, Levy YR, Friedrickson SD. Estimation of concentration of low density lipoprotein cholesterol in plasma without use of preparative ultracentrifuge. Clin Chem (1972)18: 499.
[22] Masiello P, Broca C, Gross R, Roye M, Manteghetti M, Hillaire-Buys D et al. Experimental NIDDM: Development of a new model in adult rats administered streptozotocin and nicotinamide. Diabetes (1998)47(2): 224-229.
[23] Ramachandra YL, Ashajyothi C, Padmalatha RS. Evaluation of anti-diabetic and antipyretic activity of Butea monosperma (lam) extracts on rats. Int J Pharm Phytochemical Res (2012)3: 73.
[24] Venkatesh S, Reedy GD, Reedy BM. Anti-hyperglycemic activity of Helicteres isora root in alloxan induced diabetic rats. Pharmaceu Biol (2003)41: 347.
[25] Chakravarti BK, Gupta S, Gambir SS, Gode KD. Pancreatic beta cell regeneration in rats by (−) epicatechin. Lancet (1981)2: 759.
[26] Chattopadhyay RR, Sarkar SK, Ganguly S, Banerjee RN, Basu TK. Hypoglycemic and Anti-hyperglycemic effects of leaves of Vinca rosea Linn. Indian J Physiol and Pharmacol (1991)35: 145.
[27] Sathya V, Bharathidasan R, Tamil SS, Sophia R N, Ilakkiya R, Prabakaran M. Quantitative, qualitative phytochemical analysis and in vitro antibacterial activity of Bauhinia tomentosa L. J Nat Prod Plant Resour (2013) 3: 31.
[28] Sharma SR, Dwivedi SK, Swarup D. Hypoglycaemic and hypolipidemic effects of Cinnamomum tamala Nees leaves. Indian J Exp Biol (1996)34: 372.
[29] Sharma SR, Dwivedi SK, Varshney VP, Swarup D. Anti-hyperglycemic and insulin release effects of Aegle marmelos leaves in streptozotocin-diabetic rats. Phytother Res (1996)10: 426.
[30] Chakravarti BK, Gupta S, Gambir SS, Gode KD. Pancreatic beta cell regeneration: A novel anti-diabetic mechanism of Pterocarpus marsupium Roxb. Indian J Pharmacol (1980)12: 123.
- Abstract Viewed: 142 times
- IJPS_Volume 10_Issue 1_Pages 1-14 Downloaded: 27 times