Preparation and Characterization of Thermoresponsive In-situ Forming Poloxamer Hydrogel for Controlled Release of Nile red-loaded Solid Lipid Nanoparticles Controlled release of Nile red loaded SLN by thermoresponsive sol-gel incorporation
Iranian Journal of Pharmaceutical Sciences,
دوره 9 شماره 4 (2013),
1 October 2013
,
صفحه 39-50
https://doi.org/10.22037/ijps.v9.40858
چکیده
Preparation and characterization of thermoresponsive in-situ forming poloxamer hydrogel for controlled release of Nile red-loaded solid lipid nanoparticles. Nanoparticles (NPs) are cleared rapidly from systemic circulation and do not provide sustained action in most cases. To solve this problem, this investigation introduces an erodible in-situ forming gel system as potential vehicles for prolonged release of NPs. In this study, Nile red-containing SLNs were prepared by solidification of an oil-in-water microemulsion using stearic acid, surfactants and co-surfactants. SLN particles were then loaded in a Poloxamerthermoresponsive sol-gel matrix. Dialysis membrane and membrane-less diffusion method were used to study release of the fluorescent probe. Erosion test were carried out by gravimetric method and the medium was checked for zeta potential to investigate existence of intact SLNs. Sol-gel transition temperature was determined by stirring method. Release results showed high entrapment of Nile red in lipid matrix of SLN. Therefore, Nile red content in erosion medium was attributed to SLN particles. Zeta potential of SLNs remained unchanged after sol-gel loading (P>0.05). The correlated released amount of Nile red to dissolved gel weight implied erosion could be major mechanism of SLN release. Results also showed that SLN increase erosion rate of Poloxamer gel and its sol-gel transition temperature. The present study show that thermoresponsivePoloxamer gel can be used to control the release of NPs and those intact NPs are released from this system. The prepared formulation can be used for further investigations in vivo.
- Controlled release
- Erosion
- in-situ forming sol-gel
- solid lipid nanoparticles
- Thermoresponsive.
ارجاع به مقاله
مراجع
[2] Muller R H, Mader K, and Gohla S. Solid lipid nanoparticles (SLN) for controlled drug delivery - a review of the state of the art. Eur J Pharm Biopharm (2000). 50(1): 161-77.
[3] zurMühlen A, Schwarz C, and Mehnert W. Solid lipid nanoparticles (SLN) for controlled drug delivery – Drug release and release mechanism. Eur J Pharm Biopharm (1998). 45(2): 149-155.
[4] Mehnert W and Mäder K. Solid lipid nanoparticles: production, characterization and applications,Adv Drug Deliv Rev (2001). 47(2–3): 165-196.
[5] Lin C-C and Metters A T. Hydrogels in controlled release formulations: network design and mathematical modeling. Adv Drug Deliv Rev(2006). 58(12): 1379-1408.
[6] Dumortier G, et al. A review of poloxamer 407 pharmaceutical and pharmacological characteristics.Pharm Res (2006). 23(12): 2709-28.
[7] Krezanoski J Z. Clear, water-miscible, liquid pharmaceutical vehicles and compositions which gel at body temperature for drug delivery to mucous membranes.A.U. patent 515420, April 1981
[8] Mortensen K and Pedersen J S. Structural study on the micelle formation of poly (ethylene oxide)-poly (propylene oxide)-poly (ethylene oxide) triblock copolymer in aqueous solution. Macromolecules, (1993). 26(4): 805-812.
[9] Pec E A, Wout Z G, and Johnston T P. Biological activity of urease formulated in poloxamer 407 after intraperitoneal injection in the rat. J Pharm Sci (1992). 81(7): 626-30.
[10] Wang P-L and Johnston T P. Sustained-release interleukin-2 following intramuscular injection in rats.Int J Pharm(1995). 113(1): 73-81.
[11] Barichello J M, et al. Absorption of insulin from Pluronic F-127 gels following subcutaneous administration in rats.Int J Pharm(1999). 184(2): 189-198.
[12] Kabanov A V, Batrakova E V, and Alakhov V Y. Pluronic block copolymers as novel polymer therapeutics for drug and gene delivery. J Control Release (2002). 82(2-3): 189-212.
[13]Zhang L, et al. Development and in-vitro evaluation of sustained release Poloxamer 407 (P407) gel formulations of ceftiofur. J Control Release (2002). 85(1): 73-81.
[14] Veyries M, et al. Controlled release of vancomycin from poloxamer 407 gels. Int J Pharm (1999). 192(2): 183-193.
[15] Amiji M M, et al. Intratumoral administration of paclitaxel in an in situ gelling poloxamer 407 formulation Pharm Dev Technol(2002). 7(2): 195-202.
[16] Hao J, et al. Fabrication of a composite system combining solid lipid nanoparticles and thermosensitive hydrogel for challenging ophthalmic drug delivery. Colloids Surf B Biointerfaces (2014). 114: 111-20. [17] Perinelli D, et al. Couldalbumin affect the self-assembling properties of a block co-polymer system and drug release? An in-vitro study.Pharm Res (2014). 1-11.
[18] Hyun H, et al. In vitro and in vivo release of albumin using a biodegradable MPEG-PCL diblock copolymer as an in situ gel-forming carrier.Biomacromolecules (2007). 8(4): 1093-1100.
[19] Muthu M S and Singh S. Studies on biodegradable polymeric nanoparticles of risperidone: in vitro and in vivo evaluation. Nanomedicine, (2008). 3(3): 305-319.
[20] Pan W and Yang Z. ThermoreversiblePluronic® F127-based hydrogel containing liposomes for the controlled delivery of paclitaxel: in vitro drug release, cell cytotoxicity, and uptake studies. Int J Nanomedicine (2011). 6: 151-166.
[21] Kojarunchitt T, et al. Development and characterisation of modified poloxamer 407 thermoresponsive depot systems containing cubosomes. Int J Pharm (2011). 408(1–2): 20-26.
[22] Alinaghi A, et al. Hydrogel-embeded vesicles, as a novel approach for prolonged release and delivery of liposome, in vitro and in vivo. J Liposome Res (2013). 23(3): 235-43.
[23] Greenspan P and Fowler S D. Spectrofluorometric studies of the lipid probe, Nile red. J Lipid Res (1985). 26(7): 781-9.
[24] Stuart M C, van de Pas J C, and Engberts J B. The use of Nile Red to monitor the aggregation behavior in ternary surfactant–water–organic solvent systems.J Phys Org Chem(2005). 18(9): 929-934.
[25] Greenspan P, Mayer E P, and Fowler S D. Nile red: a selective fluorescent stain for intracellular lipid droplets. J Cell Biol. (1985). 100(3): 965-973.
[26] Gasco M R. Method for producing solid lipid microspheres having a narrow size distribution. U. S. 5250236 A, October 5,1993
[27] Bocca C, et al. Phagocytic uptake of fluorescent stealth and non-stealth solid lipid nanoparticles. Int J Pharm(1998). 175(2): 185-193.
[28] Lamprecht A and Benoit J-P. Simple liquid-chromatographic method for Nile Red quantification in cell culture in spite of photobleaching.J Chromatogr B (2003). 787(2): p 415-419.
[29] Stewart J C M. Colorimetric determination of phospholipids with ammonium ferrothiocyanate. Anal Biochem(1980). 104(1): 10-14.
[30] Schmolka I R. Artificial skin. I. Preparation and properties of pluronic F-127 gels for treatment of burns. J Biomed Mater Res, (1972). 6(6): 571-82.
[31] Kim E-Y, et al. rhEGF/HP-β-CD complex in poloxamer gel for ophthalmic delivery. IntJ Pharm (2002). 233(1): 159-167.
[32] Inal O and Yapar E A. Effect of mechanical properties on the release of meloxicam from poloxamer gel bases.IndianJ Pharm Sci (2013). 75(6): 700-6.
[33] Delmas T, et al. Encapsulation and release behavior from lipid nanoparticles: Model study with Nile red fluorophore. J of Colloid Science and Biotechnology (2012). 1(1): 16-25.
[34] Dumortier G, et al. Rheological study of a thermoreversible morphine gel. Drug Dev Ind Pharm (1991). 17(9): 1255-1265.
[35] Artzner F, et al. Interactions between Poloxamers in aqueous solutions: micellization and gelation studied by differential scanning calorimetry, small angle X-ray scattering, and rheology. Langmuir, (2007). 23(9): 5085-5092.
- چکیده مشاهده شده: 104 بار
- IJPS_Volume 9_Issue 4_Pages 39-50 (English) دانلود شده: 25 بار