Influence of Cypermethrin on DNA and RNA Content in Different Organs of Fresh Water Fish Cyprinus carpio Effect of Cypermethrin on DNA and RNA in Fishes
Iranian Journal of Pharmaceutical Sciences,
مجلد 9 عدد 3 (2013),
1 July 2013
,
الصفحة 1-10
https://doi.org/10.22037/ijps.v9.40864
الملخص
In this study, freshwater fish Cyprinus carpio was exposed to sub lethal concentration of synthetic pyrethroids insecticide cypermethrin to evaluate the impact on nucleic acids in different organs. Fishes were exposed to sub lethal concentration (1/10th of LC50 value) of cypermethrin for three different durations, 7, 14 and 21 days. In seven day exposed fishes, DNA and RNA contents were not altered by cypermethrin. Whereas in 14 day and 21 day exposed fishes the DNA and RNA content decreased significantly in brain, gills and liver. However, the decrease was more in 21 day exposed fishes. The reduction in the DNA and RNA content in cypermethrin exposed fishes is comparatively less in liver when compared to the brain and gills. Cypermethrin treatment diminished RNA/DNA ratio in all the fish organs tested. In all the three organs studied, the influence of cypermethrin was found to be exposure time dependent.
- Brain
- Cypermethrin
- Cyprinus carpio
- DNA
- Gills
- Liver
- RNA
كيفية الاقتباس
المراجع
[2] Smith TM and Stratton GW. Effects of synthetic pyrethroids insecticide on non-target organisms. Resd. Rev. (1986) 97: 93-120.
[3] Oudou HC, Alonso RM and Bruun HHC. Voltametric behavior of the synthetic pyrethroids lamda cyhalothrin and its determination in soil and well water. Anal. Chem. Acta. (2004)523: 69-74.
[4] Edwards R, Millburn P, Hutson DH. Comparative toxicity of cis-cypermethrin in rainbow trout, frog, mouse and quail. Toxicol. App.l Pharmacol. (1986)84: 512-522.
[5] Clark JR, James M, Patrick J, Douglas PM, James CM. Relative sensitivity of six estuarine fishes to carbophenothion, chlorpyrifos and fenvalerate. Ecotoxicol. Environ. Saf. (1985)10: 382-390
[6] Korea Crop Production Association. Agrochemical Year Book; Moonsun Printing: Seoul, Korea (2004) 236-323.
[7] Jayswal AP. Management of American bollworm on cotton in Andhra Pradesh. Ind Farming, July (1989) 6-7.
[8] Govindassamy P, Suganthy OM, Kalaiselvi A, Gowri B and Ramalingam V. Time dependent variations of antioxidant enzyme activities in different organs of fresh water fish Cyprinus carpio exposed to synthetic pyrethroids, Cypermethrin. Am. J. Pharm. Tech. Res. (2013)3(4): 712-724.
[9] Farah MA, Atteq B, Ali MN and Ahmad W. Evaluation of genotoxicity of PCP and 2,4d by micronucleus test in freshwater fish, Channa punctatus. Ecotoxicol. Environ. Saf. (2003)54: 25-29.
[10] Handy RD, Jha AN and Depledge MH. Biomarker approaches for ecotoxicological biomonitoring at different levels of biological organization. In Burden, F., McKelview, I., Forstner, U., Guenther, A (eds). Handbook of Environmental Monitoring, MCGraw Hill, New York (2001) 9.1-9.32.
[11] Patel S, Pandey AK, Bajpayee M, Parmar D, Dhawan A. Cypermethrin-induced DNA damage in organs and tissues of the mouse: Evidence from the comet assay. Mut. Res/Gen. Toxicol. Environ. Mutgns. (2006)607(2): 176-183.
[12] APHA/AWWA/WEF. Standard methods for the examination of water and waste water, 20thEdn. American Public Health Association, New York, USA (1998) 976.
[13] Schneider WC. Determination of nucleic acid in tissue by pentose analysis. In: Colowick, SP and Kaplan NO (eds), Methods in Enzymology, Vol III, Academic press, New York (1957) 680-684.
[14] Burton KA. Study of the condition and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. J. Biochem. (1956) 62: 315-323.
[15] Cerioti G. Determination of nucleic acids in animal tissues. J. Biol. Chem. (1955)214: 59-70.
[16] Ansari BA and Kumar K. Cypermethrin toxicity: Effect on the carbohydrate metabolism of the Indian catfish, Heteropneustes fossilis. Sci. Total. Environ. (1988)72: 161-166.
[17] Achariya S, Dutta T and Das MK. Inflluence of sub lethal ammonia toxicity on some physiological parameters of Labeo rohita (Hamilton-Buchanan) fingerlings. J. Environ. Biol. (2005)26(4): 615-620.
[18] Singh SK, Singh SK and Yadav RP. Toxicological and biochemical alterations of Cypermethrin (Synthetic pyrethroids) against freshwater teleost fish Colisa fasciatus at different season. World J. Zoo. (2010)5(1): 25-32.
[19] Nordenskjold M, Soderhall J and Moldeus P. Studies on DNA strand break induced in human fibroblast by chemical mutagens and carcinogens. Mut. Res. (1979)63: 393-400.
[20] Buckley LJ. Changes in ribonucleic acid, deoxyribonucleic acid and protein content during ontogenesis in winter flounder, Psedopleuronectes americanus and effect of starvation. Fish Bull. U.S (1980)77: 703-708.
[21] Massimo M, Milena V, Scassellati SG and Rossana P. Pesticide induced primary DNA damage in peripheral blood leucocytes of farm workers evaluated by the computerized comet assay. Biomarkers (2000)5: 192-204.
[22] Vrhovae GV and Zeljezic D. Evaluation of DNA damage in workers occupationally exposed to pesticide using SCG assay. Pesticides genotoxicity revealed by comet assay. Mut. Res. (2000)469: 279-285.
[23] Mathur PC. Pesticides industry in India. Pest. Infrm. (1988)23: 17-29.
[24] Van Loon AAWM, Groenendijk RH, Van Der Shcanslohman PHM and Bran RA. Detection of induced damage in DNA in human blood exposed to ionic radiation at biologically relevant doses. Int. J. Rad. Biol. (1991)59: 651- 660.
[25] Youson JH. First metamorphosis. In: W.S. Hoar and D.J. Randall, (Eds.). Fish Physiol. (1988)Vol. II, Academic Press, New York.
[26] Heath AG. Physiology and Ecological Health. In: Cech JJ, Wilson BW, Gosby DG (Eds.). Multiple Stresses in Ecosystem. Lewis Publishers, Washington DC, USA (1998)59-89.
[27] Tiwari S and Singh A. Metabolic changes in the Snake head fish, Channa punctatus due to lattices of Euphorbia rovleana. Asian Fish. Sci. (2003)16: 147-155.
[28] Aditya AK, Chattopadhyay S and Mitra S. Effect of mercury and methyl parathion on the ovaries of Labeo rohita. J. Environ. Biol. (2002)23(1): 61-64.
[29] Tripathi G and Verma P. Endosulfan mediated biochemical changes in the fresh water fish, Clarias batrachus. Environ. Sci. (2004a)17(1): 47-56.
[30] Tripatho G and Verma P. Fenvalerate-induces changes in catfish, Clarias batrachus: metabolic enzymes, RNA and proteins. Comp. Biochem. Physiol. Toxicol. Pharmacol. (2004b)138(1): 75-79.
[31] Sheela M and Muniandi S. Impact of cypermethrin on protein conversion efficiency, RNA, protein, glycogen content and protease enzyme activity in different tissues of the fish, Lepidocephalichthyes thermalis. Environ. Ecol. (1992)10: 829-832.
[32] Durairaj S and Selvarajan VR. Influence of quinalophos on organophosphorous pesticide and the biochemical constituents on the tissue of fish, Oreochromis mossambicus. J. Environ. Biol. (1992)13(3): 181-185.
[33] Joshi VM and Desai AK. Biochemical changes in the liver of fish, Tilapia mossambica (Peters) during continuous exposure to monocrotophos. Ecotoxicol. Environ. Saf. (1998)15(3): 272-276.
[34] Khurshid A. Pyrethroids insecticide induces teratological and biochemical changes in young chick embryos. J. Biol. Sci. (2003)6(19): 1698-1705.
[35] Das BK and Mukherjee SC. Toxicity of cypermethrin in Labeo rohita fingerlings; biochemical, enzymatic and haematological consequences. Comp. Biochem. Physiol. (2003)134: 109-121.
[36] Tripathi PK, Srivastava VK and Singh A. Toxic effects of dimethoate (organophosphate) on metabolism and enzyme system on freshwater teleost fish Channa punctatus. Asian Fish. Sci. (2003)16: 349-359.
[37] Shukla V and Pandey K. Altered nucleic acid metabolism in the fresh water fish, Colisa fasciatus. Acta Hydrochem. Hydrobiol. (1983)12: 217-219.
[38] Aruoma OI, Halliwell B, Gajewski E and Dizdaroglu M. Copper ion dependent damage to the bases in DNA in the presence of H2O2. J. Biochem. (1991)273: 601-604.
[39] Rathod ND and Kshirsagar RV. Quantification of nucleic acid from fresh water fish Punctius arenatus (Day), exposed to pesticides. Int. J. Adv. Biotech. Res. (2010)1(1): 43-51.
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