Influence of Additives on Fabrication and Release from Protein Loaded Microparticles Protein loaded microparticles
Iranian Journal of Pharmaceutical Sciences,
Vol. 8 Núm. 3 (2012),
1 July 2012
,
Página 171-179
https://doi.org/10.22037/ijps.v8.40935
Resumen
The purpose of this study was to investigate the effect of additives, poly(ethylene glycol) (PEG) 1450, poloxamer 407, polyvinyl alcohol (PVA) and sodium chloride in order to improve physico-chemical characteristics, encapsulation efficiency and in vitro release of bovine serum albumin, form poly(D,L-lactic-co-glycolic acid) (PLGA) microparticles prepared by the w/o/w solvent evaporation method. The
addition of PEG 1450 and 0.05 M NaCl changed the surface characteristics of microparticles and also affected the encapsulation efficiency and burst release of protein. The effect of surfactants: polyvinyl alcohol and poloxamer 407 used in the outer water phase was investigated. The surfactant/PLGA mass ratio played an important role in the preparation procedure of the particles. This ratio was found to be approximately 0.5 for PVA and 5 for poloxamer 407 in order to achieve microparticles with narrow size distribution (<70 μm) and good encapsulation efficiency (>70%).
- Bovine serum albumin
- Encapsulation efficiency
- Initial release
- Modified Lowry Protein Assays
- Poly (lactide-co-glycolide).
Cómo citar
Citas
[2] Smith KL, Schimpf AE, Thompson KE. Biodegradable polymers for delivery of macromolecules. Adv Drug Del Rev 1990; 4: 343-57.
[3] Yang YY, Chung TS. Morphology, drug distribution, and in vitro release profiles of biodegradable polymeric microparticles containing protein fabricated by double-emulsion solvent extraction/evaporation method. Biomaterial 2001; 22: 231-41.
[4] Rajiv J. The manufacturing techniques of various drug loaded biodegradable poly (lactide-coglycolide) (PLGA) devices. Biomaterial 2000; 21: 2475-90.
[5] Brubeck KJ, Desnoyer JR, McHugh AJ. Phase inversion dynamics of PLGA solutions related to drug delivery. Part II. The role of solution thermo dynamics and bath-side mass transfer. J Control Rel 1999; 62: 333-44.
[6] Cleek RL, Ting KC, Eskin SG, Mikos AG.Microparticles of poly (DL-lactic-co-glycolic acid)/poly (ethylene glycol) blends for controlled drug delivery. J Control Rel 1997; 48: 259-68.
[7] Su Z, Sun F, Shi Y. Effects of formulation parameters on encapsulation efficiency and release behavior of risperidone poly (D,L-lactide-coglycolide) microsphere. Chem Pharm Bull (Tokyo). 2009; 57: 1251-6.
[8] Igartua M, Hernandez A, Esquisabel A. Stability of BSA encapsulated into PLGA microparticles using PAGE and capillary electrophoresis. Int J Pharm 1998; 169: 45-54.
[9] Lowry OH, Rosebrough NJ, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265-75.
[10] Korsmeyer RW, Gurny R, Peppas NA. Mechanisms of solute release from porous hydrophilic polymers. Int J Pharm 1983; 15: 25-35.
[11] Higuchi T. Mechanism of sustained-action medication: theoretical analysis of rate of release of solid drugs dispersed in solid matrices. J Pharm Sci 1963; 52: 1145-1149.
[12] Dash S, Murthy N, Nath N. Kinetic modeling on drug release from controlled drug delivery system. Acta Pol Pharm Drug Res 2010; 67: 217-23.
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