Lethality Assay of Radiopharmaceutical bis-Thiosemicarbazones Using Brine Shrimp (Artemia salina) Test Toxicity of bis-thiosemicarbazones
Iranian Journal of Pharmaceutical Sciences,
Vol. 8 No. 3 (2012),
1 Tir 2012
,
Page 197-201
https://doi.org/10.22037/ijps.v8.40942
Abstract
In the present study, aqueous solutions of some copper-complexing ligands were screened for their cytotoxicity using brine shrimp lethality test. Among the ligands tested, diacetyl-bis(N4- methylthiosemicarbazones) (ATSM) proved to be the most safe and non-toxic compound (2% lethality at 10 ppm), while pyruvaldehyde Bis(N4-methyl)thiosemicarbazone (PTSM) was shown to possess low toxicity (7% lethality at 10 ppm) and glyoxal-bis-thiosemicarbazone (GTS) proved to be a toxic compound even at 1 ppm (20% lethality). An interesting structure-toxicity relationship was observed for the ligands based on their water solubility leading to more toxicity which can be related to polysaccharide crustae of the shrimps. Considering the 1-10 ppm to be the maximum possible concentration of the ligands in the final
pharmaceutical samples, the safety of these ligands are ATSM>PTSM>GTS.
- Brine shrimp
- Cytotoxicity
- Radiopharmaceutical
- bis-Thiosemicar bazones
How to Cite
References
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