New Usage of a Fluorometric Method to Assay Antioxidant Activity in Plant Extracts Fluorometric assay for antioxidant activity assessment
Iranian Journal of Pharmaceutical Sciences,
Vol. 8 No. 1 (2012),
15 January 2012
,
Page 71-78
https://doi.org/10.22037/ijps.v8.40978
Abstract
In the present study, an oxidative conversion of non-fluorescent dichlorofluoroscein (LDCF) to fluorescent dichlorofluoroscein (DCF) was used for detection of antioxidant properties of plant extracts. The rate of the reaction was followed by monitoring the formation of DCF as a function of time. The antioxidant assay was carried out for different concentrations of gallic acid, Salvia mirzayanii, Rech. f & Esfand Phlomis persica Boiss crude extracts and their fractions obtained by thin layer chromatography (TLC). The results showed that the fluorometric method could be used to detect lower concentrations of antioxidants. Thus, we were able to identify the antioxidant activity of five fractions obtained by TLC of Salvia mirzayanii extract and four fractions in TLC of Phlomis persica extract. This method is a good candidate to be used in high throughput screening.
- Antioxidant activity
- Dichlorofluorescein
- Fluorometric method
- Phlomis persica
- Salvia mirzayanii
- Thin layer chromatography
How to Cite
References
[2] Halliwell B, Gutteridge JMC. Free radicals in biology and medicine (3rd ed). Oxford: Oxford University Press. 1999; PP. 299-315.
[3] Nakayama T, Yamada M, Osawa T, Kawakishi S.Suppression of active oxygen-induced cytotoxicity by flavonoids. Biochem Pharmacol 1993; 45:265-7.
[4] Kumar A, Chattopadhyay S. DNA damage protecting activity and antioxidant potential of Pudina extract. Food Chem 2007; 100: 1377-84.
[5] Butterfield DA, Kanski J. Brain protein oxidation in age-related neurodegenerative disorders that are associated with aggregated proteins. Aging Res Rev 2001; 122: 945-62.
[6] Markesbery WR. Oxidative stress hypothesis in Alzheimer’s disease. Free Radic Biol Med 1997;23: 134-47.
[7] Stadtman ER. Protein oxidant and aging. Science 1992; 257: 1220-4.
[8] Hempel SL, Buettner GR, Malley OYQ, Wessels DA, Flaherty DM. Dihydrofluorescein diacetate is superior for detecting intracellular oxidants:comparison with 2',7'-dichloridihydrofluoresceindiacetate, 5 (and 6)-carboxy-2',7'-dichloridihydrofluorescein diacetate, and dihydrorhodamine 123. Free Radic Biol Med 1999; 27: 146-59.
[9] Moein S, Khaghani SH, Farzami B, Moein MR.Antioxidant properties and protective effect on cell cytotoxicity of Salvia mirzayanii. Pharm Biol 2007; 45: 458-63.
[10] Moein S, Khaghani SH, Farzami B, Moein MR.Antioxidant properties and protective effect on cell cytotoxicity of Phlomis persica. Daru 2007; 15:83-7.
[11] Marini GB, Nicoletti M, Patamia M. Plant screening by chemical and chromatographic procedures under field conditions. J Chromat 1981; 213: 113-27.
[12] Farzami B, Bathaie SZ, Sadeghi R, Shamsaie A.DNA as an enzyme the effect of imidazole derivatives as a cofactors and metal ions as activators or inhibitors. Clin Biochem 2003; 36:353-8.
[13] Kestone AS, Brandt RB. The fluorometric analysis of ultramicro quantities of hydrogen peroxide.Anal Biochem 1965; 11: 1-5.
[14] Zhao Y, Hailing L, Zhonghong G, Yuefa G, Huibi Xu. Effects of flavonoids extracted from Scutellaria baicalensis Georgi hemin-nitrite-H2O2 induced liver injury. Eur J Pharmacol 2006; 536: 192-9.
[15] Thomas DD, Espey MG, Vitek MP, Miranda KM, Wink DA. Protein nitration is mediated by heme and free metals through fenton-type chemistry: an alternative to the NO/O2 reaction. Proc Natl Acad
Sci 2002; 99: 12691-6.
[16] Bian K, Gao ZH, Weisbrodt N, Murad F. The nature of heme/iron-induced protein tyrosine nitration. Proc Natl Acad Sci 2003; 100: 5712-7.
[17] Ursini F, Maiorino M, Ferri L, Valente M , Gregolin C. Hydrogen peroxide and hematin in microsomal lipid peroxidation. J Inorg Biochem 1981; 15: 163-9.
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