High Performance Liquid Chromatography Determination of Sertraline in Human Quantification of sertraline by HPLC
Iranian Journal of Pharmaceutical Sciences,
Vol. 5 No. 3 (2009),
1 July 2009
,
Page 171-178
https://doi.org/10.22037/ijps.v5.41213
Abstract
An accurate and sensitive reversed-phase high-performance liquid chromatograph-ic method for determination of sertraline in human serum is described using 4-chloro-7-nitrobenzofurazan as pre-column derivatization agent. The drug and aninternal standard (azithrimycin) were extracted from serum using a mixture ofdiethyl ether-chloroform and subjected to the pre-column derivatization with thereagent. Analysis of the resulted derivatives was performed on a Lichrosorb CN(250×4.0 mm) column using a mobile phase composed of methanol and sodium phosphate buffer (0.05 M; pH 3.7) containing 2 ml/lit triethylamine (63:37 v/v).Detector response was monitored at excitation and emission wavelengths of 470 and537 nm, respectively. The calibration curve was linear over the concentration range of 2 to 640 ng/ml. The lower limits of detection and quantification were 0.5 and 2ng/ml, respectively. The validation of the analysis was carried out in terms of specificity, sensitivity, linearity, precision, accuracy and stability. The validated method was shown to be accurate, with intra-day and inter-day accuracy from 0.3 to 4.2%and precise, with intra-day and inter-day precision from 2.4 to 15.5%. The drug is detected at concentrations as low as 2 ng/ml in a 0.5 ml serum sample and the described method can be easily applied in human single-dose pharmacokinetic studies of sertraline.
- 4-Chloro-7-nitrobenzofurazan
- Determination
- HPLC
- Sertraline.
How to Cite
References
[2]Potter, W.Z, Hollister L.E. Antidepressant agents.In. Katzung BG, (editor). Basic and clinical pharmacology. 10thed. New York: The McGraw-Hill Companies, 2007; p. 475.
[3]Mandrioli R, Saracino MA, Ferrari S, Berardi D, Kenndler E, Raggi MA. HPLC analysis of the second-generation antidepressant sertraline and its main metabolite N-desmethylsertraline in human plasma. J Chromatogr B2006; 836:116-9.
[4]Patel J, Spencer EP, Flanagan RJ. HPLC of sertraline and norsertraline in plasma or serum.Biomed Chromatogr 1996; 10: 351-4.
[5]Lucca A, Gentilini G, Lopez-Silvia S, Soldarini A. Simultaneous determination of human plasma levels of four selective serotonin reuptake inhibitors by high-performance liquid chromatography. Ther Drug Monit2000; 22:271-6.
[6]Jain DS, Sanyal M, Subbaiah G, Pande UC,Shrivastav P. Rapid and sensitive method for the determination of sertraline in human plasma using liquid chromatography-tandem mass spectrometry (LC-MS/MS). J Chromatogr B2005; 829: 69-74.
[7]Rogowsky D, Marr M, Long G, Moore C.Determination of sertraline and desmethylsertra-line in human serum using copolymeric bonded-phase extraction, liquid chromatography and gas chromatography-mass spectrometry. J Chromatogr B1994; 655: 138-41.
[8]Chen X, Duan X, Dai X, Zhong D. Development and validation of a liquid chromatographic/tandem mass spectrometric method for the determination of sertraline in human plasma. Rapid Commun Mass Spectrom 2006; 20: 2483-9.
[9]He L, Feng F, Wu J. Determination of sertralinein human plasma by high-performance liquid chromatography-elecrospray ionization mass spectrometry and method validation. J Chromatogr Sci2005; 43: 532-5.[10]Musenga A, Kenndler E, Mercolini L, Amore M, Fanali S, Raggi MA. Determination of sertraline and N-desmethylsertraline in human plasma by CE with LIF detection. Electrophore-sis2007; 28: 1823-31.[11]Kyung MK, Hwa JB, Ho CM, Soo WJ, Ki-Jung P, Chul CB. Rapid and sensitive determination of sertraline in human plasma using gas chromatography-mass spectrometry. J Chromatogr B2002; 769: 333-9.[12]Guo X, Fukushima T, Li F, Imai K. Determination of fluxetine and norfluxetine in rat plasma by HPLC with pre-column derivatization and fluorescence detection. Biomed Chromatogr2003;17: 1-5.
[13]Fukushima T, Naka-aki E, Guo X, Li F,Vankeirsbilck T, Baeyens WRG, Imai K, Toka T.Determination of fluxetine and norfluxetine in rat brain microdialysis samples following intrapritoneal fluoxetine. Analytica Chimica Acta 2004; 522: 99-104.
[14]Bahrami G, Mohammadi B. Rapid and sensitive bioanalytical method for measurement of fluvoxamine in human serum using 4-nitrobenzo-furazan as pre-column drivatization agent:Application to a human pharmacokinetic study.J Chromatogr B2007; 857: 322-6.
[15]Higashi Y, Matsumura H, Fujii Y. Determination of fluvoxamine in rat plasma by HPLC with pre-column derivatization and fluorescence detection using 4-fluoro-7-nitro-2,1,3-benzoxadiazole.Biomed Chromatogr2005; 19: 771-6.177
- Abstract Viewed: 241 times
- IJPS_Volume 5_Issue 3_Pages 171-178 Downloaded: 94 times