Anti-Alzheimer and Antioxidant Activity of Celastrus paniculatus Seed Antioxidant activity of Celastrus paniculatus
Iranian Journal of Pharmaceutical Sciences,
卷 7 编号 1 (2011),
15 January 2011
,
第 49-56 页
https://doi.org/10.22037/ijps.v7.41353
摘要
The crude methanolic extract of the seeds of Celastrus paniculatus along with its organic soluble fractions were tested for their possible antioxidant and antialzheirmer (AD) activity. The extracts showed prominent DPPH free radical scavenging activity, inhibiting activity of authentic peroxynitrite (ONOO-) and inhibition of total reactive oxygen species (ROS) generation. In DPPH radical scavenging assay, the EtOAc fraction showed the highest activity with a IC50 value of 25.92±1.02 μg/ml whereas aqueous fractions had no activity at all within the tested concentration. Scavenging of the authentic ONOO- system, all
extract/fractions showed good activity and among them, EtOAc fraction had the highest activity with a IC50 value of 15.79±0.18 μg/ml. EtOAc fraction also showed significant (p<0.001) inhibitory activity against the total ROS generation which was almost similar with that of the positive control Trolox (IC50 16.79±0.19 μg/ml). All extract/fractions exhibited statistically significant (p<0.001) cholinesterases (ChEs) inhibitory effects with IC50 values ranging between 134.7-227.5 μg/ml for AChE and 209.6-562.1 μg/ml for BChE.
- Anti-alzheimer;
- Antioxidant;
- Cholinesterase,
- Celastrus paniculatus,
- ROS
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参考
[2] Adams RL, Crai PL, Parsons OA. Neuropsychology of dementia. Neurolog Clin 1984; 4: 387-405.
[3] Aisen PS, Davis KL. The search for diseasemodifying treatment for Alzheimer’s Disease. Neurology 1997; 48: 35-41.
[4] Johnson N, Davis T, Bosanquet N. The epidemic of Alzheimer’s disease; how can we manage the costs? Pharmacogenomics 2000; 18: 215-23.
[5] Camps P, El-Achab R, Morral J, Torrero DM, Badia A, Banos JE, Vivas NM, Barril X, Orozco M, Luque FJ. New tacrine-huperzine A hybrids (huprines): highly potent tight-binding acetylcholinesterase inhibitors of interest for the treatment of Alzheimer’s disease. J Med Chem 2000; 43: 4657-66.
[6] Parihar MS, Hemnani T. Alzheimer’s disease pathogenesis and therapeutic interventions. J Clin Neurosci 2004; 11: 456-67.
[7] Enz A, Amstutz R, Boddeke H, Gmelin G, Malonowski J. Brain selective inhibition of acetylcholinesterase: a novel approach to therapy for Alzheimer’s disease. Prog Brain Res 1993; 98: 431-45.
[8] Torreilles F, Salman-Tabcheh S, Guerin M, Torreilles J. Neurodegenerative disorders: the role of peroxynitrite. Brain Res Rev 1999; 30: 153- 63.
[9] Butterfield DA, Reed T, Newman SF, Sultana R.Roles of amyloid β-peptide-associated oxidative stress and brain protein modifications in the pathogenesis of Alzheimer's disease and mild cognitive impairment. Free Rad Bio Med 2007; 43: 658-77.
[10] Markesbery WR. Oxidative stress hypothesis in Alzheimer's disease. Free Rad Bio Med 1997; 23: 134-47.
[11] Butterfield DA, Drake J, Pocernich C, Castegna A. Evidence of oxidative damage in Alzheimer's disease brain: central role for amyloid β-peptide.Trends Mol Med 2001; 7: 548-54.
[12] Tran MH, Yamada K, Nakajima A, Mizuno M, He J, Kamei H, Nabeshima T. Tyrosine nitration of a synaptic protein synaptophysin contributes to amyloid ß-peptide-induced cholinergic dysfunction. Mol Psyc 2003; 8: 407-12.
[13] Gaitonde BB, Raiker KP, Shroff FN, Patel JR.Pharmacological studies with Malkangunin indigenous tranquilizing drug. Curr Med Prac 1957; 1: 619-21.
[14] Ahmad R, Khan RA, Rasheed S. Preliminary screening of methanolic extract of Celastrus paniculatus and Tecomella undulate for analgesic and anti-inflammatory activities. J Ethnopharm 1994; 42: 193-8.
[15] Karanth KS, Padma TK, Gunasundari MN.Influence of Celastrus oil on learning and memory.Arogya 1981; 7: 83-6.
[16] Hakim RA. A trial report on Malkanguni oil with other indigenous drugs in the treatment of psychiatric cases. Gujarat State Branch of Med Bul 1964; 77-8.
[17] Nalini K, Aroor AR, Kumar RA. Studies on biogenic amines and their metabolites in mentally retarded children on Celastrus oil therapy. Alter Med 1986; 1: 355-60.
[18] Nalini K, Karanth KS, Rao A, Aroor AR. Effects of Celastrus paniculatus on passive avoidance performance and biogenic amine turnover in albino rats. J Ethnopharm 1995; 17: 101-8.
[19] Gattu M, Boss KL, Alvin V, Terry JR, Buccafusco J. Reversal of scopolamine induced deficits in navigational memory performance by the seed oil of Celastrus paniculatus. Pharm Biochem Behavior 1997; 57: 793-9.
[20] Russo A, Izzo A, Cardile V, Borelli F, Vanella A.Indian medicinal plants as antiradicals and DNA cleavage protectors. Phytomedicine 2001; 8: 125-32.
[21] Braca A, Tommasi ND, Bari LD, Pizza C, Politi M, Morelli I. Antioxidant principles from Bauhinia terapotensis. J Nat Prod 2001; 64: 892-5.
[22] Kooy NW, Royall JA, Ischiropoulos H, Beckman JS. Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free Rad Bio Med 1994; 16:149-56.
[23] Label CP, Bondy SC. Sensitive and rapid quantitation of oxygen reactive species formation in rat synaptosomes. Neurochem Inter 1990; 17:435-41.
[24] Ellman GL, Courtney D, Andress V and Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 1961; 7: 88-95.
[25] Hebert LE, Scherr PA, Beckeff LA. Age-specific incidence of Alzheimer’s disease in a community population. J Am Med Assoc 1995; 273: 1354-9.
[26] Beard CM, Kokmen E, Kurland LT. Prevalence of dementia is changing over time in Rochester, Minnesota. Neurology 1995; 45: 75-9.
[27] Arnold SE, Kumar A. Reversible dementias. Med Clin North America 1993; 77: 215-25.
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