An Improved GC Method forRapid Analysis of Valproic Acid in Human Plasma Without Derivatization Determination of valproic acid in plasma
Iranian Journal of Pharmaceutical Sciences,
Vol. 3 No. 1 (2007),
15 January 2007
,
Page 37-42
https://doi.org/10.22037/ijps.v3.40337
Abstract
Asimple, reproducible and rapid gas chromatographic method for precisedetermination of valproic acid (VPA) in human plasma has been developed. Totaltime for sample preparation and GC analysis is less than 45 min. After plasma proteinprecipitation, VPAwas extracted into chloroform with suitable recovery. By usingStabilwax®-DAcapillary GC column, a symmetrical gas chromatographic peak wasobtained without the need for derivatization. The calibration curve was proved tobe linear (r2 = 0.998) in a wide concentration range (0.45-100 μg/ml). Inter-day andintra-day accuracy and precision of this method was investigated during the methodvalidation and the method has good precision and accuracy. This method is highlyreproducible with a limit of detection 150 ng/ml of VPAin human plasma and couldbe used in TDM and pharmacokinetic studies.
- Determination
- Gas chromatography
- Human plasma
- Valproic acid
How to Cite
References
[2]Loiseau P. Rational use of valproate: Indicationsand drug regimen in epilepsy. Epilepsia1984; 25:S65-72.
[3]Dulac O, Steru D, Rey E, Perre AT, Arthuis M.Sodium valproate monotherapy in childhoodepilepsy. Brain Dev1986; 8: 47-52.
[4]Taylor WJ, Diers Caviness MH. Atextbook for theclinical application of TDM. Irving: AbbottLaboratories, 1986; pp. 114-34.
[5]Eadie MJ. Therapeutic drug monitoring-antiepileptic drugs. Br J Clin Pharmacol 1998; 46:185-93.
[6]Perucca E. Is there a role for therapeutic drugmonitoring of new anticonvulsants. ClinPharmacokinet 2000; 38: 191-204.
[7]Tomson T, Johannessen SI. Therapeuticmonitoring of the new antiepileptic drugs. Eur JClin Pharmacol2000; 55: 697-705.
[8]Wad N. Determination of theophylline in humansera with a HPLC column switching technique.J Chromatogr 1984; 305: 127-33.
[9]Lovett LJ, Nygrad GA, Erdmann GR, BurleyCZ, Khalil SKW. Determination of sodiumvalproate in tablets by HPLC. J Liq Chromatogr1987; 10: 687-99.
[10]Liu H, Forman LJ, Montoya J, Eggers C, BarhamC, Delgado M. Determination of valproic acid byhigh-performance liquid chromatography withphotodiode-array and flouresence detection. JChromatogr1992; 576: 163-73.
[11]Lucarelli C, Villa P, Lombardi E, Berga A. HPLCmethod for simultaneous analysis of valproicacid and other common anticonvulsant drugs inhuman plasma or serum. Chromatographia1992;33: 37-41.
[12]Gatti R, Carvrini V, Roveri P. 2-Bromoacetyl-6-methoxynaphtalene: Auseful fluorescent labelingreagent for HPLC analysis of carboxylic acids.Chromatographia1992; 33: 13-18.
[13]Lin W, Kelley AR. Determination of valproicacid in plasma or serum by solid-phase columnextraction and gas-liquid chromatography. TherDrug Monit 1985; 7: 336-43.
[14]Acheampong AA, Abbott FS, Orr JM, FergusenSM, Burton RW. Use of hexadeuterated valproicacid and gas chromatography-mass spectrometryto determine the pharmacokinetics of valproicacid. J Pharm Sci1984; 73: 489-94.
[15]Thormann W, Theurillat R, Wind M, Kuldvee R.Therapeutic drug monitoring of antiepileptics bycapillary electrophoresis: Characterization ofassays via analysis of quality control seracontaining 14 analytes. J Chromatogr2001; 924:429-37.
[16]Chollet DF. Determination of antiepileptic arugsin biological material. J Chromatogr B AnalytTechnol Biomed Life Sci 2002; 767: 191-233.
- Abstract Viewed: 95 times
- IJPS_Volume 3_Issue 1_Pages 37-42 Downloaded: 14 times